miRNA display CGI


Results 1 - 8 of 8 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
32178 3' -53.9 NC_007016.1 + 31402 0.66 0.941397
Target:  5'- -cAUCGGGgGGCugucaaACACGUCGUcaaacgaugGAGUCg -3'
miRNA:   3'- ucUAGCCCgUCG------UGUGCGGUA---------UUCAG- -5'
32178 3' -53.9 NC_007016.1 + 114545 0.66 0.920191
Target:  5'- aAGGg-GGGCAGUucguugagACGCGUCAcGAGUCc -3'
miRNA:   3'- -UCUagCCCGUCG--------UGUGCGGUaUUCAG- -5'
32178 3' -53.9 NC_007016.1 + 108903 0.66 0.914249
Target:  5'- --uUCGGGCAGCAgggacucaaACGCCAaUAAaucGUCa -3'
miRNA:   3'- ucuAGCCCGUCGUg--------UGCGGU-AUU---CAG- -5'
32178 3' -53.9 NC_007016.1 + 128900 0.67 0.908053
Target:  5'- ----gGGGCAGuUACACGCCAUGu--- -3'
miRNA:   3'- ucuagCCCGUC-GUGUGCGGUAUucag -5'
32178 3' -53.9 NC_007016.1 + 16660 0.67 0.901603
Target:  5'- cGAaCGGGCGGCACACGgUcc-GGUUc -3'
miRNA:   3'- uCUaGCCCGUCGUGUGCgGuauUCAG- -5'
32178 3' -53.9 NC_007016.1 + 39106 0.7 0.748512
Target:  5'- -cAUCGGGUuGCACAUGCCAc--GUCc -3'
miRNA:   3'- ucUAGCCCGuCGUGUGCGGUauuCAG- -5'
32178 3' -53.9 NC_007016.1 + 122715 0.71 0.718056
Target:  5'- gAGuUCGGGCGGCACGuacgcccacUGCCAgGGGUg -3'
miRNA:   3'- -UCuAGCCCGUCGUGU---------GCGGUaUUCAg -5'
32178 3' -53.9 NC_007016.1 + 84098 0.9 0.057945
Target:  5'- cAGAU-GGcGCAGCACACGCCAUAAGUCu -3'
miRNA:   3'- -UCUAgCC-CGUCGUGUGCGGUAUUCAG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.