Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 322 | 3' | -47 | AC_000010.1 | + | 12700 | 0.66 | 0.959074 |
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Target: 5'- ----aCCUGAAUgGUCuguGGGGAGUGCg -3' miRNA: 3'- gugugGGGCUUA-CAGuu-UCUUUCACG- -5' |
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| 322 | 3' | -47 | AC_000010.1 | + | 26365 | 0.67 | 0.939392 |
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Target: 5'- -uCACCCCGug-GUgGAAGAgcAGGUaGCg -3' miRNA: 3'- guGUGGGGCuuaCAgUUUCU--UUCA-CG- -5' |
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| 322 | 3' | -47 | AC_000010.1 | + | 34844 | 0.68 | 0.914471 |
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Target: 5'- -cCugCCUG-GUGUUGGAGAGAGUGa -3' miRNA: 3'- guGugGGGCuUACAGUUUCUUUCACg -5' |
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| 322 | 3' | -47 | AC_000010.1 | + | 2066 | 0.69 | 0.884305 |
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Target: 5'- gACACCcaCCGAccAUGcCAgcgguuuugGAGGAGGUGCa -3' miRNA: 3'- gUGUGG--GGCU--UACaGU---------UUCUUUCACG- -5' |
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| 322 | 3' | -47 | AC_000010.1 | + | 11772 | 0.7 | 0.849201 |
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Target: 5'- gCugACCuuGAGUGaCGAucuGGGAGUGUa -3' miRNA: 3'- -GugUGGggCUUACaGUUu--CUUUCACG- -5' |
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| 322 | 3' | -47 | AC_000010.1 | + | 11382 | 0.71 | 0.809743 |
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Target: 5'- gCGCACCCUGAuUGcCcgcGAGGAGGUGa -3' miRNA: 3'- -GUGUGGGGCUuACaGu--UUCUUUCACg -5' |
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| 322 | 3' | -47 | AC_000010.1 | + | 11131 | 0.71 | 0.799287 |
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Target: 5'- uGCACCCgCGggUGccgcUCGGGcGAuGGUGCa -3' miRNA: 3'- gUGUGGG-GCuuAC----AGUUU-CUuUCACG- -5' |
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| 322 | 3' | -47 | AC_000010.1 | + | 27652 | 0.72 | 0.755509 |
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Target: 5'- aCGCACUCCGugggccgGUCccuGAGGGUGCu -3' miRNA: 3'- -GUGUGGGGCuua----CAGuuuCUUUCACG- -5' |
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| 322 | 3' | -47 | AC_000010.1 | + | 15165 | 0.73 | 0.661666 |
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Target: 5'- -cCACCUCGGAUGUCAccuguggagcaGAGcAGGUGUa -3' miRNA: 3'- guGUGGGGCUUACAGU-----------UUCuUUCACG- -5' |
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| 322 | 3' | -47 | AC_000010.1 | + | 18850 | 1.14 | 0.002139 |
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Target: 5'- aCACACCCCGAAUGUCAAAGAAAGUGCu -3' miRNA: 3'- -GUGUGGGGCUUACAGUUUCUUUCACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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