Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32202 | 3' | -52.8 | NC_007016.1 | + | 88671 | 0.66 | 0.948619 |
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Target: 5'- aUAGGGAUcugcaguguggaCGCCAGCAUaACGUG-UCg- -3' miRNA: 3'- -AUCCCUA------------GCGGUUGUG-UGCACuAGag -5' |
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| 32202 | 3' | -52.8 | NC_007016.1 | + | 85250 | 0.67 | 0.944068 |
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Target: 5'- cGGGcacuggcuaGAUCGCCGACucCugGcGAUCUCc -3' miRNA: 3'- aUCC---------CUAGCGGUUGu-GugCaCUAGAG- -5' |
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| 32202 | 3' | -52.8 | NC_007016.1 | + | 18880 | 0.67 | 0.923291 |
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Target: 5'- -cGGGGUCGCgCGuuucgcaaACACACGaGGUUUCc -3' miRNA: 3'- auCCCUAGCG-GU--------UGUGUGCaCUAGAG- -5' |
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| 32202 | 3' | -52.8 | NC_007016.1 | + | 87831 | 0.67 | 0.923291 |
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Target: 5'- --aGGAUCGCacaaAACGCuguGCGUGGUUUCc -3' miRNA: 3'- aucCCUAGCGg---UUGUG---UGCACUAGAG- -5' |
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| 32202 | 3' | -52.8 | NC_007016.1 | + | 72662 | 0.68 | 0.904987 |
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Target: 5'- gAGGGugcUGCCGAguugUACGCGgGAUCUCa -3' miRNA: 3'- aUCCCua-GCGGUU----GUGUGCaCUAGAG- -5' |
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| 32202 | 3' | -52.8 | NC_007016.1 | + | 72372 | 0.69 | 0.877041 |
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Target: 5'- gGGGGAUgGUCAugGCAUGUGGc--- -3' miRNA: 3'- aUCCCUAgCGGUugUGUGCACUagag -5' |
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| 32202 | 3' | -52.8 | NC_007016.1 | + | 103690 | 0.69 | 0.845307 |
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Target: 5'- cAGGGG-CGCCAGCAgAgcCGUGAcgCUCc -3' miRNA: 3'- aUCCCUaGCGGUUGUgU--GCACUa-GAG- -5' |
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| 32202 | 3' | -52.8 | NC_007016.1 | + | 103469 | 0.7 | 0.819275 |
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Target: 5'- gUGGGGcgCGCUGccGCGCACGUGAa--- -3' miRNA: 3'- -AUCCCuaGCGGU--UGUGUGCACUagag -5' |
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| 32202 | 3' | -52.8 | NC_007016.1 | + | 106818 | 1.09 | 0.004214 |
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Target: 5'- aUAGGGAUCGCCAACACACGUGAUCUCg -3' miRNA: 3'- -AUCCCUAGCGGUUGUGUGCACUAGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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