Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32211 | 3' | -47.6 | NC_007016.1 | + | 65489 | 0.67 | 0.997188 |
Target: 5'- gAACUGGCgugcgGCUGUCAGa--GCUGCa- -3' miRNA: 3'- -UUGAUUGa----CGACAGUCcaaUGAUGag -5' |
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32211 | 3' | -47.6 | NC_007016.1 | + | 122901 | 0.69 | 0.984997 |
Target: 5'- cGCU-GCUGCUGguUCAGG-UGgUACUCa -3' miRNA: 3'- uUGAuUGACGAC--AGUCCaAUgAUGAG- -5' |
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32211 | 3' | -47.6 | NC_007016.1 | + | 79018 | 0.73 | 0.911544 |
Target: 5'- --aUGGCUGUauaUGUCAGGUUGaUACUCa -3' miRNA: 3'- uugAUUGACG---ACAGUCCAAUgAUGAG- -5' |
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32211 | 3' | -47.6 | NC_007016.1 | + | 112371 | 1.08 | 0.014328 |
Target: 5'- cAACUAACUGCUGUCAGGUUACUACUCu -3' miRNA: 3'- -UUGAUUGACGACAGUCCAAUGAUGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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