miRNA: 3'- -UAGUCACAGg-----CAGCGUCU------GGGaCAau -5'
miRNA: 3'- uagUCACAGGCAgCGUCUGgGACAau -5'
miRNA: 3'- uaGUCACAGGCAGCGUCUGGGACAAU- -5'
miRNA: 3'- -UAGUCACAGGCAGCGUCUGGGACAAU- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32212 | 3' | -54.8 | NC_007016.1 | + | 7364 | 0.66 | 0.897147 |
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Target: 5'- cGUCGGUGUCgaagaaGUCGCAGAacauguCCCgGUg- -3' miRNA: 3'- -UAGUCACAGg-----CAGCGUCU------GGGaCAau -5' |
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| 32212 | 3' | -54.8 | NC_007016.1 | + | 80924 | 0.66 | 0.875861 |
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Target: 5'- --gAGUGUCUGUgGCAGGCgUUGUc- -3' miRNA: 3'- uagUCACAGGCAgCGUCUGgGACAau -5' |
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| 32212 | 3' | -54.8 | NC_007016.1 | + | 113164 | 0.97 | 0.016459 |
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Target: 5'- -aCAGUGUCCGUUGCAGACCCUGUUAg -3' miRNA: 3'- uaGUCACAGGCAGCGUCUGGGACAAU- -5' |
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| 32212 | 3' | -54.8 | NC_007016.1 | + | 113236 | 1.05 | 0.004804 |
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Target: 5'- aAUCAGUGUCCGUCGCAGACCCUGUUAa -3' miRNA: 3'- -UAGUCACAGGCAGCGUCUGGGACAAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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