Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32214 | 5' | -59.2 | NC_007016.1 | + | 114786 | 0.66 | 0.711895 |
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Target: 5'- cUCGGCGGGuCUG-AGGUaUGGGgaaUCCc -3' miRNA: 3'- aAGUCGCCCuGACaUCCG-ACCC---AGGa -5' |
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| 32214 | 5' | -59.2 | NC_007016.1 | + | 62941 | 0.67 | 0.661068 |
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Target: 5'- aUCGGCaacauccuGGGAgUGgccGGCgGGGUCCUu -3' miRNA: 3'- aAGUCG--------CCCUgACau-CCGaCCCAGGA- -5' |
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| 32214 | 5' | -59.2 | NC_007016.1 | + | 131207 | 0.68 | 0.558633 |
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Target: 5'- -cCGGCGGucCUGgggaGGGCUcGGGUCCc -3' miRNA: 3'- aaGUCGCCcuGACa---UCCGA-CCCAGGa -5' |
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| 32214 | 5' | -59.2 | NC_007016.1 | + | 821 | 0.68 | 0.558633 |
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Target: 5'- -cCGGCGGucCUGgggaGGGCUcGGGUCCc -3' miRNA: 3'- aaGUCGCCcuGACa---UCCGA-CCCAGGa -5' |
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| 32214 | 5' | -59.2 | NC_007016.1 | + | 68614 | 0.69 | 0.508894 |
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Target: 5'- --gGGgGGGAUUGUAGGCcauauaGGUCCUg -3' miRNA: 3'- aagUCgCCCUGACAUCCGac----CCAGGA- -5' |
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| 32214 | 5' | -59.2 | NC_007016.1 | + | 114662 | 1.05 | 0.001993 |
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Target: 5'- aUUCAGCGGGACUGUAGGCUGGGUCCUc -3' miRNA: 3'- -AAGUCGCCCUGACAUCCGACCCAGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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