Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32215 | 3' | -46.2 | NC_007016.1 | + | 6743 | 0.66 | 0.999931 |
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Target: 5'- aGGGGUCCCUgc-GCuGAGggGUGGUUAc -3' miRNA: 3'- -CCCUAGGGGgauUG-UUCuaCAUUAAU- -5' |
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| 32215 | 3' | -46.2 | NC_007016.1 | + | 27484 | 0.69 | 0.997709 |
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Target: 5'- cGGGA-CCCCCUGcugGAGAUGUu---- -3' miRNA: 3'- -CCCUaGGGGGAUug-UUCUACAuuaau -5' |
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| 32215 | 3' | -46.2 | NC_007016.1 | + | 114572 | 0.73 | 0.970467 |
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Target: 5'- gGGGAUUCCCCaAACAAGGUa------ -3' miRNA: 3'- -CCCUAGGGGGaUUGUUCUAcauuaau -5' |
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| 32215 | 3' | -46.2 | NC_007016.1 | + | 23223 | 0.76 | 0.89304 |
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Target: 5'- uGGGAUUCCCUUAACAGGGaagGUAu--- -3' miRNA: 3'- -CCCUAGGGGGAUUGUUCUa--CAUuaau -5' |
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| 32215 | 3' | -46.2 | NC_007016.1 | + | 114791 | 0.83 | 0.549525 |
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Target: 5'- gGGGAUUCCCCUAACAAGGuauaacuaaUGUAAa-- -3' miRNA: 3'- -CCCUAGGGGGAUUGUUCU---------ACAUUaau -5' |
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| 32215 | 3' | -46.2 | NC_007016.1 | + | 115131 | 0.92 | 0.205545 |
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Target: 5'- gGGGAUUCCCCUAACAGGGUGaAAUUAu -3' miRNA: 3'- -CCCUAGGGGGAUUGUUCUACaUUAAU- -5' |
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| 32215 | 3' | -46.2 | NC_007016.1 | + | 114934 | 1.11 | 0.016826 |
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Target: 5'- gGGGAUCCCCCUAACAAGAUGUAAUUAa -3' miRNA: 3'- -CCCUAGGGGGAUUGUUCUACAUUAAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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