Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32215 | 5' | -45.1 | NC_007016.1 | + | 118742 | 0.66 | 0.999961 |
Target: 5'- --------gGCCCgGUcgAGGGGggUCg -3' miRNA: 3'- auauuaaagUGGGaCAa-UCCCCuuAG- -5' |
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32215 | 5' | -45.1 | NC_007016.1 | + | 117181 | 0.72 | 0.984208 |
Target: 5'- -----gUUC-CCCUGUUGGGGGGc-- -3' miRNA: 3'- auauuaAAGuGGGACAAUCCCCUuag -5' |
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32215 | 5' | -45.1 | NC_007016.1 | + | 114732 | 0.75 | 0.942941 |
Target: 5'- aUGUGGgcUUUACCUUGUUuGGGGAAUCc -3' miRNA: 3'- -AUAUUa-AAGUGGGACAAuCCCCUUAG- -5' |
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32215 | 5' | -45.1 | NC_007016.1 | + | 115092 | 0.82 | 0.666927 |
Target: 5'- -uUAAUUaCAUCUUGUUAGGGGGAUCc -3' miRNA: 3'- auAUUAAaGUGGGACAAUCCCCUUAG- -5' |
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32215 | 5' | -45.1 | NC_007016.1 | + | 114949 | 0.85 | 0.510067 |
Target: 5'- -uUAGUUaUACCUUGUUAGGGGAAUCc -3' miRNA: 3'- auAUUAAaGUGGGACAAUCCCCUUAG- -5' |
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32215 | 5' | -45.1 | NC_007016.1 | + | 114971 | 1.09 | 0.02586 |
Target: 5'- aUAUAAUUUCACCCUGUUAGGGGAAUCc -3' miRNA: 3'- -AUAUUAAAGUGGGACAAUCCCCUUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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