Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32221 | 3' | -51.3 | NC_007016.1 | + | 100613 | 0.67 | 0.976765 |
Target: 5'- aGGGGguuggaacgUGUCcagggcUGUGAGCACggcgccaaagaauagUUCCUCCu -3' miRNA: 3'- -CCCU---------ACAGa-----ACACUCGUGa--------------AAGGGGG- -5' |
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32221 | 3' | -51.3 | NC_007016.1 | + | 116439 | 0.74 | 0.71434 |
Target: 5'- -cGAUGUCUUGccAGCACUUUCCUguCCa -3' miRNA: 3'- ccCUACAGAACacUCGUGAAAGGG--GG- -5' |
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32221 | 3' | -51.3 | NC_007016.1 | + | 121184 | 0.78 | 0.529509 |
Target: 5'- cGGGUGUUUuuuuguauaggucuaUGUGAGCGCgcguggCCCCCg -3' miRNA: 3'- cCCUACAGA---------------ACACUCGUGaaa---GGGGG- -5' |
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32221 | 3' | -51.3 | NC_007016.1 | + | 116246 | 1.16 | 0.002757 |
Target: 5'- gGGGAUGUCUUGUGAGCACUUUCCCCCg -3' miRNA: 3'- -CCCUACAGAACACUCGUGAAAGGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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