Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32244 | 3' | -46.9 | NC_007022.1 | + | 56197 | 0.66 | 0.999928 |
Target: 5'- uGCCUCCGAUU-GAaaCACGAGgaccCCAAAu -3' miRNA: 3'- -UGGAGGCUAAgCUaaGUGUUU----GGUUU- -5' |
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32244 | 3' | -46.9 | NC_007022.1 | + | 24868 | 0.67 | 0.999731 |
Target: 5'- cACCUCCGGauguugaaUUCGA-UCugAAAgCCGAAg -3' miRNA: 3'- -UGGAGGCU--------AAGCUaAGugUUU-GGUUU- -5' |
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32244 | 3' | -46.9 | NC_007022.1 | + | 52687 | 0.68 | 0.999325 |
Target: 5'- uCCaggCCGGUUCGAUgaacCGCGuACCAGAu -3' miRNA: 3'- uGGa--GGCUAAGCUAa---GUGUuUGGUUU- -5' |
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32244 | 3' | -46.9 | NC_007022.1 | + | 67866 | 0.7 | 0.993966 |
Target: 5'- aACCUCCGAcuUUCuggUCcCAAACCAGAc -3' miRNA: 3'- -UGGAGGCU--AAGcuaAGuGUUUGGUUU- -5' |
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32244 | 3' | -46.9 | NC_007022.1 | + | 128569 | 0.71 | 0.987779 |
Target: 5'- gGCCUgUGggUCGAUgCACGAACCGc- -3' miRNA: 3'- -UGGAgGCuaAGCUAaGUGUUUGGUuu -5' |
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32244 | 3' | -46.9 | NC_007022.1 | + | 41679 | 0.73 | 0.972048 |
Target: 5'- uUCUuuGAUUCGAUUCAU-AACCAGc -3' miRNA: 3'- uGGAggCUAAGCUAAGUGuUUGGUUu -5' |
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32244 | 3' | -46.9 | NC_007022.1 | + | 5275 | 1.07 | 0.030306 |
Target: 5'- aACCUCCGAUUCGAUUCACAAACCAAAg -3' miRNA: 3'- -UGGAGGCUAAGCUAAGUGUUUGGUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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