miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
32244 3' -46.9 NC_007022.1 + 56197 0.66 0.999928
Target:  5'- uGCCUCCGAUU-GAaaCACGAGgaccCCAAAu -3'
miRNA:   3'- -UGGAGGCUAAgCUaaGUGUUU----GGUUU- -5'
32244 3' -46.9 NC_007022.1 + 24868 0.67 0.999731
Target:  5'- cACCUCCGGauguugaaUUCGA-UCugAAAgCCGAAg -3'
miRNA:   3'- -UGGAGGCU--------AAGCUaAGugUUU-GGUUU- -5'
32244 3' -46.9 NC_007022.1 + 52687 0.68 0.999325
Target:  5'- uCCaggCCGGUUCGAUgaacCGCGuACCAGAu -3'
miRNA:   3'- uGGa--GGCUAAGCUAa---GUGUuUGGUUU- -5'
32244 3' -46.9 NC_007022.1 + 67866 0.7 0.993966
Target:  5'- aACCUCCGAcuUUCuggUCcCAAACCAGAc -3'
miRNA:   3'- -UGGAGGCU--AAGcuaAGuGUUUGGUUU- -5'
32244 3' -46.9 NC_007022.1 + 128569 0.71 0.987779
Target:  5'- gGCCUgUGggUCGAUgCACGAACCGc- -3'
miRNA:   3'- -UGGAgGCuaAGCUAaGUGUUUGGUuu -5'
32244 3' -46.9 NC_007022.1 + 41679 0.73 0.972048
Target:  5'- uUCUuuGAUUCGAUUCAU-AACCAGc -3'
miRNA:   3'- uGGAggCUAAGCUAAGUGuUUGGUUu -5'
32244 3' -46.9 NC_007022.1 + 5275 1.07 0.030306
Target:  5'- aACCUCCGAUUCGAUUCACAAACCAAAg -3'
miRNA:   3'- -UGGAGGCUAAGCUAAGUGUUUGGUUU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.