Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32246 | 3' | -47.8 | NC_007022.1 | + | 128978 | 0.66 | 0.999676 |
Target: 5'- cGUcGGCCuucaguuGaGCGAAAGCCGgGAAAa -3' miRNA: 3'- aCAaUUGGu------C-CGCUUUCGGCgUUUUc -5' |
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32246 | 3' | -47.8 | NC_007022.1 | + | 104147 | 0.66 | 0.999627 |
Target: 5'- aGUgGACCccgcugcuggaAGGCGAAGGCCugccggaaauugcugGCAAAAa -3' miRNA: 3'- aCAaUUGG-----------UCCGCUUUCGG---------------CGUUUUc -5' |
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32246 | 3' | -47.8 | NC_007022.1 | + | 168265 | 0.66 | 0.999591 |
Target: 5'- ---gAGCCAGGUGA-AGCgGCAu--- -3' miRNA: 3'- acaaUUGGUCCGCUuUCGgCGUuuuc -5' |
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32246 | 3' | -47.8 | NC_007022.1 | + | 125494 | 0.66 | 0.999475 |
Target: 5'- ---aAGCCAGGCGAgcgugaaGAGCgGUggauAAAAGg -3' miRNA: 3'- acaaUUGGUCCGCU-------UUCGgCG----UUUUC- -5' |
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32246 | 3' | -47.8 | NC_007022.1 | + | 127759 | 0.67 | 0.99936 |
Target: 5'- -uUUAACCagauGGGCGAuAGCCGUc---- -3' miRNA: 3'- acAAUUGG----UCCGCUuUCGGCGuuuuc -5' |
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32246 | 3' | -47.8 | NC_007022.1 | + | 99541 | 0.67 | 0.999024 |
Target: 5'- --cUGGCCAcGGCGguAGCCGgCuGGAGa -3' miRNA: 3'- acaAUUGGU-CCGCuuUCGGC-GuUUUC- -5' |
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32246 | 3' | -47.8 | NC_007022.1 | + | 39371 | 0.68 | 0.997473 |
Target: 5'- ---gAAUCGGGUGAAuugaauGGCCGCAAc-- -3' miRNA: 3'- acaaUUGGUCCGCUU------UCGGCGUUuuc -5' |
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32246 | 3' | -47.8 | NC_007022.1 | + | 99477 | 0.69 | 0.993329 |
Target: 5'- ---aAACCu-GCGAuaucAGCCGCAAGAGg -3' miRNA: 3'- acaaUUGGucCGCUu---UCGGCGUUUUC- -5' |
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32246 | 3' | -47.8 | NC_007022.1 | + | 26750 | 0.7 | 0.992282 |
Target: 5'- --aUGGCCAGaGCGAAagcAGCUGCccaGAAAGa -3' miRNA: 3'- acaAUUGGUC-CGCUU---UCGGCG---UUUUC- -5' |
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32246 | 3' | -47.8 | NC_007022.1 | + | 47072 | 0.7 | 0.986733 |
Target: 5'- gUGUUcaAACCguaaAGGUGAGAGCCGUgcAGGc -3' miRNA: 3'- -ACAA--UUGG----UCCGCUUUCGGCGuuUUC- -5' |
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32246 | 3' | -47.8 | NC_007022.1 | + | 36509 | 0.7 | 0.986563 |
Target: 5'- -aUUAGCCAGGCGAAaagcaguGGCCGa----- -3' miRNA: 3'- acAAUUGGUCCGCUU-------UCGGCguuuuc -5' |
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32246 | 3' | -47.8 | NC_007022.1 | + | 133635 | 0.71 | 0.980863 |
Target: 5'- cUGUgaUGACCAuGCGAAAGCUGCcGGAu -3' miRNA: 3'- -ACA--AUUGGUcCGCUUUCGGCGuUUUc -5' |
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32246 | 3' | -47.8 | NC_007022.1 | + | 52891 | 0.72 | 0.967067 |
Target: 5'- ---cGAUUAGGCGAuAGUCGCGAAAu -3' miRNA: 3'- acaaUUGGUCCGCUuUCGGCGUUUUc -5' |
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32246 | 3' | -47.8 | NC_007022.1 | + | 157647 | 0.73 | 0.959949 |
Target: 5'- --aUGGCCAGGCGGAuGGCUGCu---- -3' miRNA: 3'- acaAUUGGUCCGCUU-UCGGCGuuuuc -5' |
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32246 | 3' | -47.8 | NC_007022.1 | + | 26470 | 1.1 | 0.017162 |
Target: 5'- aUGUUAACCAGGCGAAAGCCGCAAAAGc -3' miRNA: 3'- -ACAAUUGGUCCGCUUUCGGCGUUUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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