Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32247 | 3' | -54.3 | NC_007022.1 | + | 124368 | 0.66 | 0.962208 |
Target: 5'- gGCGGCgGUCGuuuCAuUGUAUugCCGGaGUu -3' miRNA: 3'- aCGCCG-CAGUu--GU-ACAUGugGGUC-CG- -5' |
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32247 | 3' | -54.3 | NC_007022.1 | + | 98609 | 0.66 | 0.950807 |
Target: 5'- aGCGGCGcUCAGCAgacgauuUACCCGuucGGUg -3' miRNA: 3'- aCGCCGC-AGUUGUacau---GUGGGU---CCG- -5' |
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32247 | 3' | -54.3 | NC_007022.1 | + | 90333 | 0.66 | 0.949131 |
Target: 5'- aGUGGCGUCAugGUugucgcaucaucaGUcccagagguugcuuGCGCCUGGGUc -3' miRNA: 3'- aCGCCGCAGUugUA-------------CA--------------UGUGGGUCCG- -5' |
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32247 | 3' | -54.3 | NC_007022.1 | + | 96956 | 0.67 | 0.927117 |
Target: 5'- -cCGGCGUCcuGACGagauuacugguUGUAucgcCAUCCAGGCa -3' miRNA: 3'- acGCCGCAG--UUGU-----------ACAU----GUGGGUCCG- -5' |
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32247 | 3' | -54.3 | NC_007022.1 | + | 61102 | 0.68 | 0.910015 |
Target: 5'- gGCGGCGUuucaCAAgGUGUugAaCCCuuGGUg -3' miRNA: 3'- aCGCCGCA----GUUgUACAugU-GGGu-CCG- -5' |
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32247 | 3' | -54.3 | NC_007022.1 | + | 16272 | 0.68 | 0.909408 |
Target: 5'- aGCGG-GUCGugAUGUGggacaagUGCCCAuGGCc -3' miRNA: 3'- aCGCCgCAGUugUACAU-------GUGGGU-CCG- -5' |
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32247 | 3' | -54.3 | NC_007022.1 | + | 50343 | 0.68 | 0.906335 |
Target: 5'- gGCGGUGUUaAGCAUuUACAacgcgccagcuaguuCCUAGGCg -3' miRNA: 3'- aCGCCGCAG-UUGUAcAUGU---------------GGGUCCG- -5' |
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32247 | 3' | -54.3 | NC_007022.1 | + | 90088 | 0.71 | 0.794629 |
Target: 5'- cGCGGCa-CAAUgcGUAUuucgACCCAGGCg -3' miRNA: 3'- aCGCCGcaGUUGuaCAUG----UGGGUCCG- -5' |
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32247 | 3' | -54.3 | NC_007022.1 | + | 32246 | 0.71 | 0.785464 |
Target: 5'- aGCGG-GUCGc---GUAgGCCCAGGCg -3' miRNA: 3'- aCGCCgCAGUuguaCAUgUGGGUCCG- -5' |
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32247 | 3' | -54.3 | NC_007022.1 | + | 149460 | 0.73 | 0.657005 |
Target: 5'- gGCGGCGgugcuaCAuUAUGggGCugCCAGGCa -3' miRNA: 3'- aCGCCGCa-----GUuGUACa-UGugGGUCCG- -5' |
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32247 | 3' | -54.3 | NC_007022.1 | + | 58118 | 1.13 | 0.002687 |
Target: 5'- cUGCGGCGUCAACAUGUACACCCAGGCa -3' miRNA: 3'- -ACGCCGCAGUUGUACAUGUGGGUCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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