Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32250 | 5' | -44.3 | NC_007022.1 | + | 162203 | 0.66 | 0.999999 |
Target: 5'- uGAUugGGUUGcuAUugaccAACGCCUGCAAa-- -3' miRNA: 3'- -CUGugCCGAU--UA-----UUGUGGAUGUUaaa -5' |
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32250 | 5' | -44.3 | NC_007022.1 | + | 74372 | 0.66 | 0.999999 |
Target: 5'- cGGCACcuucagcaGGCUGA--ACACCaACGAUUUg -3' miRNA: 3'- -CUGUG--------CCGAUUauUGUGGaUGUUAAA- -5' |
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32250 | 5' | -44.3 | NC_007022.1 | + | 37196 | 0.66 | 0.999999 |
Target: 5'- uACAUGGCUGAcGGCGCCga-AGUa- -3' miRNA: 3'- cUGUGCCGAUUaUUGUGGaugUUAaa -5' |
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32250 | 5' | -44.3 | NC_007022.1 | + | 15263 | 0.66 | 0.999999 |
Target: 5'- aACAauUGGCU---GACGCCUACAAa-- -3' miRNA: 3'- cUGU--GCCGAuuaUUGUGGAUGUUaaa -5' |
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32250 | 5' | -44.3 | NC_007022.1 | + | 132424 | 0.66 | 0.999998 |
Target: 5'- -uCGCGGCUGGUuGCugCaUGCAAa-- -3' miRNA: 3'- cuGUGCCGAUUAuUGugG-AUGUUaaa -5' |
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32250 | 5' | -44.3 | NC_007022.1 | + | 168381 | 0.66 | 0.999998 |
Target: 5'- -cCACGGUUGGUcucuugguCACCUACGAa-- -3' miRNA: 3'- cuGUGCCGAUUAuu------GUGGAUGUUaaa -5' |
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32250 | 5' | -44.3 | NC_007022.1 | + | 105172 | 0.67 | 0.999997 |
Target: 5'- -gUugGGCUGGUGAauCCUACAAa-- -3' miRNA: 3'- cuGugCCGAUUAUUguGGAUGUUaaa -5' |
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32250 | 5' | -44.3 | NC_007022.1 | + | 723 | 0.67 | 0.999995 |
Target: 5'- cAUACGGU----GACACCUACGAc-- -3' miRNA: 3'- cUGUGCCGauuaUUGUGGAUGUUaaa -5' |
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32250 | 5' | -44.3 | NC_007022.1 | + | 47274 | 0.67 | 0.999986 |
Target: 5'- uGCACGGCUcu---CACCUuuACGGUUUg -3' miRNA: 3'- cUGUGCCGAuuauuGUGGA--UGUUAAA- -5' |
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32250 | 5' | -44.3 | NC_007022.1 | + | 156606 | 0.68 | 0.999972 |
Target: 5'- uGACgACGGCgauuuguAUACCUACGAUa- -3' miRNA: 3'- -CUG-UGCCGauuau--UGUGGAUGUUAaa -5' |
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32250 | 5' | -44.3 | NC_007022.1 | + | 153003 | 0.69 | 0.999878 |
Target: 5'- aGCGCGuGC----AACACCUGCAGUUa -3' miRNA: 3'- cUGUGC-CGauuaUUGUGGAUGUUAAa -5' |
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32250 | 5' | -44.3 | NC_007022.1 | + | 149138 | 0.69 | 0.999878 |
Target: 5'- uGAUACGGUUGAagauauUGGCGCUUGCGGa-- -3' miRNA: 3'- -CUGUGCCGAUU------AUUGUGGAUGUUaaa -5' |
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32250 | 5' | -44.3 | NC_007022.1 | + | 107388 | 0.7 | 0.999447 |
Target: 5'- aGCACGGCUAAUGguAUGCCgucACGGUa- -3' miRNA: 3'- cUGUGCCGAUUAU--UGUGGa--UGUUAaa -5' |
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32250 | 5' | -44.3 | NC_007022.1 | + | 54688 | 0.71 | 0.998688 |
Target: 5'- aACACGGaUGAUAccuuCACCUACAAUg- -3' miRNA: 3'- cUGUGCCgAUUAUu---GUGGAUGUUAaa -5' |
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32250 | 5' | -44.3 | NC_007022.1 | + | 72051 | 0.72 | 0.997182 |
Target: 5'- aGAUaaAUGGCUAGUGgcagccaaGCGCCUGCAAUc- -3' miRNA: 3'- -CUG--UGCCGAUUAU--------UGUGGAUGUUAaa -5' |
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32250 | 5' | -44.3 | NC_007022.1 | + | 81557 | 0.77 | 0.945284 |
Target: 5'- aACAUGGCgacGAUAACACCUGCuGUUa -3' miRNA: 3'- cUGUGCCGa--UUAUUGUGGAUGuUAAa -5' |
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32250 | 5' | -44.3 | NC_007022.1 | + | 102515 | 1.06 | 0.056282 |
Target: 5'- cGACACGGCUAAUAACACCUACAAUUUc -3' miRNA: 3'- -CUGUGCCGAUUAUUGUGGAUGUUAAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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