Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32251 | 5' | -48.3 | NC_007022.1 | + | 110094 | 0.66 | 0.999721 |
Target: 5'- -aCCAACGUCAcGCcGGUCAGu---- -3' miRNA: 3'- gcGGUUGCAGUaCGaCCAGUUuuagu -5' |
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32251 | 5' | -48.3 | NC_007022.1 | + | 105023 | 0.66 | 0.999721 |
Target: 5'- gGCUAACGaagUUAUGCUGG---AAAUCAa -3' miRNA: 3'- gCGGUUGC---AGUACGACCaguUUUAGU- -5' |
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32251 | 5' | -48.3 | NC_007022.1 | + | 87110 | 0.66 | 0.999638 |
Target: 5'- gCGCCGGCGauaacguUUAUGCUgaGGUCA-AGUCc -3' miRNA: 3'- -GCGGUUGC-------AGUACGA--CCAGUuUUAGu -5' |
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32251 | 5' | -48.3 | NC_007022.1 | + | 138291 | 0.66 | 0.999445 |
Target: 5'- gCGUCGACGUCggGUUGGgucucccauUCAAcAUCGc -3' miRNA: 3'- -GCGGUUGCAGuaCGACC---------AGUUuUAGU- -5' |
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32251 | 5' | -48.3 | NC_007022.1 | + | 79612 | 0.67 | 0.99873 |
Target: 5'- -cCCAu--UCAUGCUGGgCAGAGUCAg -3' miRNA: 3'- gcGGUugcAGUACGACCaGUUUUAGU- -5' |
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32251 | 5' | -48.3 | NC_007022.1 | + | 30921 | 0.67 | 0.998461 |
Target: 5'- aUGCCucaaGUgAUGaCUGGUCAAAGUUc -3' miRNA: 3'- -GCGGuug-CAgUAC-GACCAGUUUUAGu -5' |
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32251 | 5' | -48.3 | NC_007022.1 | + | 47587 | 0.67 | 0.998461 |
Target: 5'- uCGCCuGACGguauccgAUGCUGGUCAGAu--- -3' miRNA: 3'- -GCGG-UUGCag-----UACGACCAGUUUuagu -5' |
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32251 | 5' | -48.3 | NC_007022.1 | + | 140583 | 0.68 | 0.996284 |
Target: 5'- uCGCCGagaugaauagcaACuUCAUGCUGGUCAcggcGAAUUu -3' miRNA: 3'- -GCGGU------------UGcAGUACGACCAGU----UUUAGu -5' |
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32251 | 5' | -48.3 | NC_007022.1 | + | 105236 | 0.68 | 0.995634 |
Target: 5'- uCGCCAAacuggcCGUgGUGCUGG-CAAcuUCAu -3' miRNA: 3'- -GCGGUU------GCAgUACGACCaGUUuuAGU- -5' |
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32251 | 5' | -48.3 | NC_007022.1 | + | 35012 | 0.7 | 0.989529 |
Target: 5'- aGCCGAUGa---GCUGGUCAAAAUg- -3' miRNA: 3'- gCGGUUGCaguaCGACCAGUUUUAgu -5' |
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32251 | 5' | -48.3 | NC_007022.1 | + | 104344 | 0.72 | 0.969918 |
Target: 5'- -aCCAACaUCGcUGCUGGUCAAAGUUc -3' miRNA: 3'- gcGGUUGcAGU-ACGACCAGUUUUAGu -5' |
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32251 | 5' | -48.3 | NC_007022.1 | + | 9282 | 0.74 | 0.921115 |
Target: 5'- gCGCCGAUGgcauUCAUGCUGGUgUAAAGUUc -3' miRNA: 3'- -GCGGUUGC----AGUACGACCA-GUUUUAGu -5' |
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32251 | 5' | -48.3 | NC_007022.1 | + | 171881 | 0.76 | 0.832684 |
Target: 5'- aCGCCAaaauGCGUUGUGCUGGUUauGAAGUUc -3' miRNA: 3'- -GCGGU----UGCAGUACGACCAG--UUUUAGu -5' |
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32251 | 5' | -48.3 | NC_007022.1 | + | 155042 | 1.09 | 0.016444 |
Target: 5'- uCGCCAACGUCAUGCUGGUCAAAAUCAc -3' miRNA: 3'- -GCGGUUGCAGUACGACCAGUUUUAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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