Results 1 - 9 of 9 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32252 | 3' | -49.5 | NC_007022.1 | + | 157877 | 0.66 | 0.998648 |
Target: 5'- --cUCGCGAaAUCccgcgaaaauGAACU-GCGCCGGc -3' miRNA: 3'- gauAGCGCUaUAG----------CUUGAuCGCGGUC- -5' |
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32252 | 3' | -49.5 | NC_007022.1 | + | 75977 | 0.68 | 0.993878 |
Target: 5'- ----aGCGAagcUCGAACUGGCgaaGCCAGu -3' miRNA: 3'- gauagCGCUau-AGCUUGAUCG---CGGUC- -5' |
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32252 | 3' | -49.5 | NC_007022.1 | + | 27658 | 0.69 | 0.987864 |
Target: 5'- --uUUGCGAUaaGUCGGACUgAGCaGCCAc -3' miRNA: 3'- gauAGCGCUA--UAGCUUGA-UCG-CGGUc -5' |
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32252 | 3' | -49.5 | NC_007022.1 | + | 136173 | 0.69 | 0.986243 |
Target: 5'- --cUCGUGGUAUCGAGaaaUGGUGCUg- -3' miRNA: 3'- gauAGCGCUAUAGCUUg--AUCGCGGuc -5' |
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32252 | 3' | -49.5 | NC_007022.1 | + | 29750 | 0.69 | 0.98446 |
Target: 5'- ----aGCGAagcUCGAACUGGCgaaGCCAGg -3' miRNA: 3'- gauagCGCUau-AGCUUGAUCG---CGGUC- -5' |
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32252 | 3' | -49.5 | NC_007022.1 | + | 149856 | 0.69 | 0.98446 |
Target: 5'- ----aGCGAagcUCGAACUGGCgaaGCCAGg -3' miRNA: 3'- gauagCGCUau-AGCUUGAUCG---CGGUC- -5' |
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32252 | 3' | -49.5 | NC_007022.1 | + | 16268 | 0.71 | 0.955885 |
Target: 5'- -gGUCGUGAUGUgGGACaAGUGCCc- -3' miRNA: 3'- gaUAGCGCUAUAgCUUGaUCGCGGuc -5' |
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32252 | 3' | -49.5 | NC_007022.1 | + | 167364 | 0.74 | 0.880764 |
Target: 5'- aUAUCGCGGUGgcaaauguucugguUCcggguGAACUGGCGCUAGu -3' miRNA: 3'- gAUAGCGCUAU--------------AG-----CUUGAUCGCGGUC- -5' |
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32252 | 3' | -49.5 | NC_007022.1 | + | 167597 | 1.09 | 0.01409 |
Target: 5'- uCUAUCGCGAUAUCGAACUAGCGCCAGu -3' miRNA: 3'- -GAUAGCGCUAUAGCUUGAUCGCGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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