miRNA display CGI


Results 1 - 11 of 11 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
32252 5' -50.2 NC_007022.1 + 172126 0.66 0.995516
Target:  5'- uGACCAGGucuuuguaucGCAgggUGCCGCCuuCGAUAa -3'
miRNA:   3'- -UUGGUCU----------UGUaa-ACGGUGGc-GCUAUa -5'
32252 5' -50.2 NC_007022.1 + 127761 0.66 0.994754
Target:  5'- uAACCAGAugGgcgauagccGUCACCGCGGc-- -3'
miRNA:   3'- -UUGGUCUugUaaa------CGGUGGCGCUaua -5'
32252 5' -50.2 NC_007022.1 + 78508 0.66 0.994754
Target:  5'- gAGCCA--GCAUccGCCuauuCCGCGGUAUa -3'
miRNA:   3'- -UUGGUcuUGUAaaCGGu---GGCGCUAUA- -5'
32252 5' -50.2 NC_007022.1 + 31780 0.66 0.992918
Target:  5'- cACCAGAGCg---GCCaguauaACCGCGAa-- -3'
miRNA:   3'- uUGGUCUUGuaaaCGG------UGGCGCUaua -5'
32252 5' -50.2 NC_007022.1 + 104469 0.66 0.991825
Target:  5'- uGACCAaaagcuucGAucaGUUUGUCAUCGCGGUAUa -3'
miRNA:   3'- -UUGGU--------CUug-UAAACGGUGGCGCUAUA- -5'
32252 5' -50.2 NC_007022.1 + 159596 0.67 0.990602
Target:  5'- cACCGGAGCAUUUcguugaGUgGCUGCGAa-- -3'
miRNA:   3'- uUGGUCUUGUAAA------CGgUGGCGCUaua -5'
32252 5' -50.2 NC_007022.1 + 107281 0.67 0.990602
Target:  5'- aAACCAuuGACAUUaaugucgGCCACCaGCGAUGUu -3'
miRNA:   3'- -UUGGUc-UUGUAAa------CGGUGG-CGCUAUA- -5'
32252 5' -50.2 NC_007022.1 + 104546 0.68 0.982201
Target:  5'- cACCAGAAC-UUUGaCCAgCaGCGAUGUu -3'
miRNA:   3'- uUGGUCUUGuAAAC-GGUgG-CGCUAUA- -5'
32252 5' -50.2 NC_007022.1 + 94988 0.68 0.97683
Target:  5'- uAUCGGAACAUccugaaaauaugguUUGCCAUCGCGcUGUc -3'
miRNA:   3'- uUGGUCUUGUA--------------AACGGUGGCGCuAUA- -5'
32252 5' -50.2 NC_007022.1 + 111798 0.69 0.962317
Target:  5'- uAACCGGGAUuucuuCCACCGUGAUAg -3'
miRNA:   3'- -UUGGUCUUGuaaacGGUGGCGCUAUa -5'
32252 5' -50.2 NC_007022.1 + 167562 1.03 0.020547
Target:  5'- gAACCAGAACAUUUGCCACCGCGAUAUu -3'
miRNA:   3'- -UUGGUCUUGUAAACGGUGGCGCUAUA- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.