Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32254 | 3' | -49.5 | NC_007022.1 | + | 81076 | 0.66 | 0.997776 |
Target: 5'- --uGAAGGUgaaACaucaGUGCAAAUUGCCGg -3' miRNA: 3'- cggUUUCCG---UGg---UACGUUUAACGGUg -5' |
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32254 | 3' | -49.5 | NC_007022.1 | + | 13981 | 0.66 | 0.997345 |
Target: 5'- cGCCAAAGGUugCuugauUGU--GUUGCUggGCa -3' miRNA: 3'- -CGGUUUCCGugGu----ACGuuUAACGG--UG- -5' |
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32254 | 3' | -49.5 | NC_007022.1 | + | 68652 | 0.66 | 0.996847 |
Target: 5'- cGCCGuaAAGGUGgCGUGCug---GCCAUa -3' miRNA: 3'- -CGGU--UUCCGUgGUACGuuuaaCGGUG- -5' |
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32254 | 3' | -49.5 | NC_007022.1 | + | 148794 | 0.67 | 0.994872 |
Target: 5'- gGCCcGAGGUggGCCAUGaauuGAGUcGCCAUc -3' miRNA: 3'- -CGGuUUCCG--UGGUACg---UUUAaCGGUG- -5' |
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32254 | 3' | -49.5 | NC_007022.1 | + | 103655 | 0.67 | 0.994025 |
Target: 5'- aGCCAGuuGGGUuUC-UGCAGAgcUUGCCAUa -3' miRNA: 3'- -CGGUU--UCCGuGGuACGUUU--AACGGUG- -5' |
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32254 | 3' | -49.5 | NC_007022.1 | + | 52064 | 0.67 | 0.994025 |
Target: 5'- cGCCAGAaGCuuAUCAUGCAuucAUUGCgGCu -3' miRNA: 3'- -CGGUUUcCG--UGGUACGUu--UAACGgUG- -5' |
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32254 | 3' | -49.5 | NC_007022.1 | + | 39034 | 0.68 | 0.986322 |
Target: 5'- aCCAAGGGCAaggaaauuUGCugaagauGUUGCCGCg -3' miRNA: 3'- cGGUUUCCGUggu-----ACGuu-----UAACGGUG- -5' |
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32254 | 3' | -49.5 | NC_007022.1 | + | 32732 | 0.69 | 0.976457 |
Target: 5'- uGCCAGaAGGCAUCGgccagaugcguaauuUGUuccAUUGCCGCu -3' miRNA: 3'- -CGGUU-UCCGUGGU---------------ACGuu-UAACGGUG- -5' |
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32254 | 3' | -49.5 | NC_007022.1 | + | 26442 | 0.69 | 0.975402 |
Target: 5'- uGCCAucGaGCGCC-UGCAAGcUGCuCGCc -3' miRNA: 3'- -CGGUuuC-CGUGGuACGUUUaACG-GUG- -5' |
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32254 | 3' | -49.5 | NC_007022.1 | + | 104245 | 0.7 | 0.969605 |
Target: 5'- gGCCAGguAGGCgGCCAUGgGAucauUUGCCuGCu -3' miRNA: 3'- -CGGUU--UCCG-UGGUACgUUu---AACGG-UG- -5' |
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32254 | 3' | -49.5 | NC_007022.1 | + | 72069 | 0.7 | 0.969605 |
Target: 5'- aGCCAA--GCGCC-UGCAAucaUGCCAUa -3' miRNA: 3'- -CGGUUucCGUGGuACGUUua-ACGGUG- -5' |
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32254 | 3' | -49.5 | NC_007022.1 | + | 163766 | 0.7 | 0.966366 |
Target: 5'- uUCAAAGGCACCGauUGCAAuacccAUUGCg-- -3' miRNA: 3'- cGGUUUCCGUGGU--ACGUU-----UAACGgug -5' |
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32254 | 3' | -49.5 | NC_007022.1 | + | 98165 | 0.7 | 0.962892 |
Target: 5'- cUCAuAGGCAggaccCCAUGCGAAUuUGCCGa -3' miRNA: 3'- cGGUuUCCGU-----GGUACGUUUA-ACGGUg -5' |
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32254 | 3' | -49.5 | NC_007022.1 | + | 69044 | 0.71 | 0.946516 |
Target: 5'- uGCCAGAGGCGa---GCGGGUUacaGCCGCu -3' miRNA: 3'- -CGGUUUCCGUgguaCGUUUAA---CGGUG- -5' |
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32254 | 3' | -49.5 | NC_007022.1 | + | 158928 | 0.71 | 0.941778 |
Target: 5'- uUCGAAGGUGCCAgUGUAuucGUUGCCAa -3' miRNA: 3'- cGGUUUCCGUGGU-ACGUu--UAACGGUg -5' |
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32254 | 3' | -49.5 | NC_007022.1 | + | 115022 | 0.72 | 0.924265 |
Target: 5'- uGCCAcAAGGUACUacucucugcccagcGUGCAAAaUGCCAg -3' miRNA: 3'- -CGGU-UUCCGUGG--------------UACGUUUaACGGUg -5' |
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32254 | 3' | -49.5 | NC_007022.1 | + | 13450 | 1.13 | 0.006251 |
Target: 5'- cGCCAAAGGCACCAUGCAAAUUGCCACu -3' miRNA: 3'- -CGGUUUCCGUGGUACGUUUAACGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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