Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32254 | 5' | -57.3 | NC_007022.1 | + | 120823 | 0.66 | 0.879829 |
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Target: 5'- aGGagAGAACCCUgCCGGGggUUCUacUCCGc -3' miRNA: 3'- aCCg-UCUUGGGAaGGCCC--AGGA--AGGU- -5' |
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| 32254 | 5' | -57.3 | NC_007022.1 | + | 4571 | 0.66 | 0.857911 |
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Target: 5'- gGGUaaauAGAACCUUgaccaUCCGGGUCagcaUCCGu -3' miRNA: 3'- aCCG----UCUUGGGA-----AGGCCCAGga--AGGU- -5' |
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| 32254 | 5' | -57.3 | NC_007022.1 | + | 150600 | 0.68 | 0.763694 |
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Target: 5'- cGGCAGGACCUUUCa-GGUUCgccCCGa -3' miRNA: 3'- aCCGUCUUGGGAAGgcCCAGGaa-GGU- -5' |
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| 32254 | 5' | -57.3 | NC_007022.1 | + | 154271 | 0.69 | 0.725438 |
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Target: 5'- --aCGGAGCCCUUCgGGGcUCCUUUgGa -3' miRNA: 3'- accGUCUUGGGAAGgCCC-AGGAAGgU- -5' |
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| 32254 | 5' | -57.3 | NC_007022.1 | + | 138186 | 0.73 | 0.496155 |
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Target: 5'- gUGGCAGAGCCauagcaucgauauCUUCCGGGU---UCCAa -3' miRNA: 3'- -ACCGUCUUGG-------------GAAGGCCCAggaAGGU- -5' |
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| 32254 | 5' | -57.3 | NC_007022.1 | + | 13484 | 1.08 | 0.002753 |
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Target: 5'- gUGGCAGAACCCUUCCGGGUCCUUCCAc -3' miRNA: 3'- -ACCGUCUUGGGAAGGCCCAGGAAGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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