Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32255 | 5' | -45.8 | NC_007022.1 | + | 21400 | 0.66 | 0.999991 |
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Target: 5'- gGCAAGCCCGAAGcaaag-UUUC-CCAg -3' miRNA: 3'- -CGUUCGGGCUUUagaaagAAAGaGGU- -5' |
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| 32255 | 5' | -45.8 | NC_007022.1 | + | 153259 | 0.67 | 0.99994 |
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Target: 5'- aGCAAGUCCGAA--------UUCUCCAg -3' miRNA: 3'- -CGUUCGGGCUUuagaaagaAAGAGGU- -5' |
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| 32255 | 5' | -45.8 | NC_007022.1 | + | 89269 | 0.68 | 0.99982 |
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Target: 5'- gGCAAG-CCGAGuugCUUaCUUUUUCCGg -3' miRNA: 3'- -CGUUCgGGCUUua-GAAaGAAAGAGGU- -5' |
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| 32255 | 5' | -45.8 | NC_007022.1 | + | 9044 | 0.69 | 0.999624 |
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Target: 5'- -uGAGCCUGAuuuUCaUUUCagUUCUCCAu -3' miRNA: 3'- cgUUCGGGCUuu-AG-AAAGa-AAGAGGU- -5' |
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| 32255 | 5' | -45.8 | NC_007022.1 | + | 115496 | 0.73 | 0.99007 |
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Target: 5'- aGCGAGCuCCGAGGUuCUUuuaaUCUcaUCUCCAc -3' miRNA: 3'- -CGUUCG-GGCUUUA-GAA----AGAa-AGAGGU- -5' |
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| 32255 | 5' | -45.8 | NC_007022.1 | + | 50260 | 1.13 | 0.019017 |
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Target: 5'- gGCAAGCCCGAAAUCUUUCUUUCUCCAa -3' miRNA: 3'- -CGUUCGGGCUUUAGAAAGAAAGAGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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