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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32256 | 3' | -48.1 | NC_007022.1 | + | 38881 | 0.66 | 0.999868 |
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Target: 5'- --cGGUguuaucCCaGGUGUUACGGAuaaAGAUUGGu -3' miRNA: 3'- agaCCA------GG-CCACAAUGUCU---UUUGACC- -5' |
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| 32256 | 3' | -48.1 | NC_007022.1 | + | 46721 | 0.67 | 0.999561 |
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Target: 5'- -aUGG-CCGGUGUuaaaauUGCuGAGuuuGCUGGu -3' miRNA: 3'- agACCaGGCCACA------AUGuCUUu--UGACC- -5' |
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| 32256 | 3' | -48.1 | NC_007022.1 | + | 150557 | 0.67 | 0.999561 |
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Target: 5'- gCUGGUauGGUGaccaaUACAGuucGACUGGa -3' miRNA: 3'- aGACCAggCCACa----AUGUCuu-UUGACC- -5' |
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| 32256 | 3' | -48.1 | NC_007022.1 | + | 31967 | 0.67 | 0.999322 |
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Target: 5'- aCUGGccgcUCUGGUGggGCuGAugGAGCUGa -3' miRNA: 3'- aGACC----AGGCCACaaUGuCU--UUUGACc -5' |
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| 32256 | 3' | -48.1 | NC_007022.1 | + | 62133 | 0.68 | 0.998189 |
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Target: 5'- cUCUGGUCCGGU--------AAGCUGGc -3' miRNA: 3'- -AGACCAGGCCAcaaugucuUUUGACC- -5' |
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| 32256 | 3' | -48.1 | NC_007022.1 | + | 141801 | 0.68 | 0.997832 |
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Target: 5'- aUUGG-CUGGUGUauCAGAuucgAAGCUGGu -3' miRNA: 3'- aGACCaGGCCACAauGUCU----UUUGACC- -5' |
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| 32256 | 3' | -48.1 | NC_007022.1 | + | 61904 | 1.14 | 0.010817 |
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Target: 5'- aUCUGGUCCGGUGUUACAGAAAACUGGg -3' miRNA: 3'- -AGACCAGGCCACAAUGUCUUUUGACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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