miRNA: 3'- gcGGUCGAA--------UGGCCUGGUCUcu--UCCa -5'
miRNA: 3'- gCGGuCGaaUGGCCUg---GUCUCUUCCA- -5'
miRNA: 3'- -GCGGUCGAAUGGCCUGGUCUCUUCCA- -5'
Results 1 - 3 of 3 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32256 | 5' | -55.9 | NC_007022.1 | + | 62104 | 0.66 | 0.933001 |
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Target: 5'- -cCCAGUUUucuguaacACCGGACCAGAucguGGGa -3' miRNA: 3'- gcGGUCGAA--------UGGCCUGGUCUcu--UCCa -5' |
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| 32256 | 5' | -55.9 | NC_007022.1 | + | 31292 | 0.67 | 0.885319 |
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Target: 5'- uGCCuGCc-GCUGGAauggCGGAGAGGGUg -3' miRNA: 3'- gCGGuCGaaUGGCCUg---GUCUCUUCCA- -5' |
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| 32256 | 5' | -55.9 | NC_007022.1 | + | 61939 | 1.07 | 0.004601 |
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Target: 5'- uCGCCAGCUUACCGGACCAGAGAAGGUc -3' miRNA: 3'- -GCGGUCGAAUGGCCUGGUCUCUUCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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