Results 1 - 20 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32257 | 3' | -45.4 | NC_007022.1 | + | 154467 | 0.66 | 0.99999 |
Target: 5'- ---cCAAAGG-AGCC-CCGAAG-GGCu -3' miRNA: 3'- uuauGUUUUUaUCGGaGGCUUCuCCG- -5' |
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32257 | 3' | -45.4 | NC_007022.1 | + | 65465 | 0.66 | 0.99999 |
Target: 5'- uAAUGCGcAGGAUGGCC----AAGAGGCa -3' miRNA: 3'- -UUAUGU-UUUUAUCGGaggcUUCUCCG- -5' |
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32257 | 3' | -45.4 | NC_007022.1 | + | 4416 | 0.68 | 0.999914 |
Target: 5'- --gAUGAAAA-GGCCUCUucGGAGGCc -3' miRNA: 3'- uuaUGUUUUUaUCGGAGGcuUCUCCG- -5' |
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32257 | 3' | -45.4 | NC_007022.1 | + | 29879 | 0.68 | 0.999851 |
Target: 5'- uAUACGAcAAaGGCCUCacuuGGAGGCc -3' miRNA: 3'- uUAUGUUuUUaUCGGAGgcu-UCUCCG- -5' |
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32257 | 3' | -45.4 | NC_007022.1 | + | 41880 | 0.68 | 0.999806 |
Target: 5'- ---cCGAA---AGcCCUCCGAAGAGGg -3' miRNA: 3'- uuauGUUUuuaUC-GGAGGCUUCUCCg -5' |
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32257 | 3' | -45.4 | NC_007022.1 | + | 25550 | 0.68 | 0.99975 |
Target: 5'- ---uCGAAuaucuGCCUCCGAAaAGGCa -3' miRNA: 3'- uuauGUUUuuau-CGGAGGCUUcUCCG- -5' |
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32257 | 3' | -45.4 | NC_007022.1 | + | 154638 | 0.69 | 0.99968 |
Target: 5'- uGGUACGGGucguGCUUCUcauaGAGGAGGCg -3' miRNA: 3'- -UUAUGUUUuuauCGGAGG----CUUCUCCG- -5' |
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32257 | 3' | -45.4 | NC_007022.1 | + | 10964 | 0.69 | 0.999594 |
Target: 5'- --gACAAAAAUAGUUUuuGggGAaGCa -3' miRNA: 3'- uuaUGUUUUUAUCGGAggCuuCUcCG- -5' |
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32257 | 3' | -45.4 | NC_007022.1 | + | 30090 | 0.69 | 0.999488 |
Target: 5'- --aGCAAGGAagaaGGCCUCCaaguGAGGCc -3' miRNA: 3'- uuaUGUUUUUa---UCGGAGGcuu-CUCCG- -5' |
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32257 | 3' | -45.4 | NC_007022.1 | + | 42902 | 0.69 | 0.999488 |
Target: 5'- cAAUGCAuucgcgcuuAGAGUGGCgaCCGucGAGGUg -3' miRNA: 3'- -UUAUGU---------UUUUAUCGgaGGCuuCUCCG- -5' |
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32257 | 3' | -45.4 | NC_007022.1 | + | 93047 | 0.69 | 0.999359 |
Target: 5'- aAAUACGAAAAaGGCUcCCGAAGGaGCc -3' miRNA: 3'- -UUAUGUUUUUaUCGGaGGCUUCUcCG- -5' |
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32257 | 3' | -45.4 | NC_007022.1 | + | 166639 | 0.7 | 0.999013 |
Target: 5'- --gACAAA---GGCCUCUucGGAGGCc -3' miRNA: 3'- uuaUGUUUuuaUCGGAGGcuUCUCCG- -5' |
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32257 | 3' | -45.4 | NC_007022.1 | + | 82371 | 0.7 | 0.999013 |
Target: 5'- cGUGCAAAAAUAGUaucUUCCGuugGGAGaGCu -3' miRNA: 3'- uUAUGUUUUUAUCG---GAGGCu--UCUC-CG- -5' |
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32257 | 3' | -45.4 | NC_007022.1 | + | 34738 | 0.72 | 0.994881 |
Target: 5'- uAAU-CAAAAcgGGCCUCUccGGAGGCc -3' miRNA: 3'- -UUAuGUUUUuaUCGGAGGcuUCUCCG- -5' |
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32257 | 3' | -45.4 | NC_007022.1 | + | 67015 | 0.72 | 0.994011 |
Target: 5'- uGUugGcccuUGGCCUCCGAcGAGGUu -3' miRNA: 3'- uUAugUuuuuAUCGGAGGCUuCUCCG- -5' |
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32257 | 3' | -45.4 | NC_007022.1 | + | 28458 | 0.73 | 0.989282 |
Target: 5'- uAUACAAAAAUAGgCUCCGuAGAGc- -3' miRNA: 3'- uUAUGUUUUUAUCgGAGGCuUCUCcg -5' |
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32257 | 3' | -45.4 | NC_007022.1 | + | 133916 | 0.74 | 0.979767 |
Target: 5'- --aACAAAAAUGcCCUCCGuAGAGGg -3' miRNA: 3'- uuaUGUUUUUAUcGGAGGCuUCUCCg -5' |
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32257 | 3' | -45.4 | NC_007022.1 | + | 63926 | 0.74 | 0.974573 |
Target: 5'- aAAUGCcugaaguuAAAUAGCCUCUucGGAGGCu -3' miRNA: 3'- -UUAUGuu------UUUAUCGGAGGcuUCUCCG- -5' |
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32257 | 3' | -45.4 | NC_007022.1 | + | 15912 | 0.78 | 0.903178 |
Target: 5'- --aACAAAAAgacCCUCCGAAGAGGg -3' miRNA: 3'- uuaUGUUUUUaucGGAGGCUUCUCCg -5' |
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32257 | 3' | -45.4 | NC_007022.1 | + | 171599 | 0.8 | 0.803166 |
Target: 5'- -uUACAAAAA-GGcCCUCCGAAGAGGg -3' miRNA: 3'- uuAUGUUUUUaUC-GGAGGCUUCUCCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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