Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 32257 | 3' | -45.4 | NC_007022.1 | + | 93047 | 0.69 | 0.999359 |
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Target: 5'- aAAUACGAAAAaGGCUcCCGAAGGaGCc -3' miRNA: 3'- -UUAUGUUUUUaUCGGaGGCUUCUcCG- -5' |
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| 32257 | 3' | -45.4 | NC_007022.1 | + | 82371 | 0.7 | 0.999013 |
|
Target: 5'- cGUGCAAAAAUAGUaucUUCCGuugGGAGaGCu -3' miRNA: 3'- uUAUGUUUUUAUCG---GAGGCu--UCUC-CG- -5' |
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| 32257 | 3' | -45.4 | NC_007022.1 | + | 166639 | 0.7 | 0.999013 |
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Target: 5'- --gACAAA---GGCCUCUucGGAGGCc -3' miRNA: 3'- uuaUGUUUuuaUCGGAGGcuUCUCCG- -5' |
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| 32257 | 3' | -45.4 | NC_007022.1 | + | 34738 | 0.72 | 0.994881 |
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Target: 5'- uAAU-CAAAAcgGGCCUCUccGGAGGCc -3' miRNA: 3'- -UUAuGUUUUuaUCGGAGGcuUCUCCG- -5' |
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| 32257 | 3' | -45.4 | NC_007022.1 | + | 67015 | 0.72 | 0.994011 |
|
Target: 5'- uGUugGcccuUGGCCUCCGAcGAGGUu -3' miRNA: 3'- uUAugUuuuuAUCGGAGGCUuCUCCG- -5' |
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| 32257 | 3' | -45.4 | NC_007022.1 | + | 63926 | 0.74 | 0.974573 |
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Target: 5'- aAAUGCcugaaguuAAAUAGCCUCUucGGAGGCu -3' miRNA: 3'- -UUAUGuu------UUUAUCGGAGGcuUCUCCG- -5' |
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| 32257 | 3' | -45.4 | NC_007022.1 | + | 154467 | 0.66 | 0.99999 |
|
Target: 5'- ---cCAAAGG-AGCC-CCGAAG-GGCu -3' miRNA: 3'- uuauGUUUUUaUCGGaGGCUUCuCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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