miRNA: 3'- -GUAUaauUC-UCUUAGaCUUCUUAACc -5'
miRNA: 3'- guauaauuCUCUUAGACUU---------------CUUAACC- -5'
miRNA: 3'- guauaAUUCUCUUAGACUUcUUAACc -5'
miRNA: 3'- -GUAUAAUUCUCUUAGACUUCUUAACC- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32258 | 3' | -37.6 | NC_007022.1 | + | 89687 | 0.67 | 1 |
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Target: 5'- aCAUGgguAGcAGAAUC-GAGGAAUUGa -3' miRNA: 3'- -GUAUaauUC-UCUUAGaCUUCUUAACc -5' |
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| 32258 | 3' | -37.6 | NC_007022.1 | + | 169481 | 0.68 | 1 |
|
Target: 5'- -------cGAGGAUCUGAGcugcgaggguaugacGGAUUGGg -3' miRNA: 3'- guauaauuCUCUUAGACUU---------------CUUAACC- -5' |
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| 32258 | 3' | -37.6 | NC_007022.1 | + | 67782 | 0.75 | 1 |
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Target: 5'- ----cUAAGAGAAUCUGAAaAAUUGu -3' miRNA: 3'- guauaAUUCUCUUAGACUUcUUAACc -5' |
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| 32258 | 3' | -37.6 | NC_007022.1 | + | 67689 | 1.17 | 0.104745 |
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Target: 5'- aCAUAUUAAGAGAAUCUGAAGAAUUGGg -3' miRNA: 3'- -GUAUAAUUCUCUUAGACUUCUUAACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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