Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32259 | 3' | -48.2 | NC_007022.1 | + | 123103 | 0.66 | 0.999639 |
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Target: 5'- uUCGCGGuUCAAUgAGAuAUCUGAcgACGAa -3' miRNA: 3'- cAGUGCC-AGUUAgUCU-UAGGCU--UGUU- -5' |
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| 32259 | 3' | -48.2 | NC_007022.1 | + | 79603 | 0.66 | 0.999295 |
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Target: 5'- -gCugGG-CAgaGUCAGAGUCCGuguGCAu -3' miRNA: 3'- caGugCCaGU--UAGUCUUAGGCu--UGUu -5' |
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| 32259 | 3' | -48.2 | NC_007022.1 | + | 6499 | 0.67 | 0.99913 |
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Target: 5'- aGUUuCGGUCuuUCAGGucgugaagCCGAACAAu -3' miRNA: 3'- -CAGuGCCAGuuAGUCUua------GGCUUGUU- -5' |
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| 32259 | 3' | -48.2 | NC_007022.1 | + | 155656 | 0.68 | 0.997278 |
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Target: 5'- -cCAUGGUgcCAGUCAGGAUaacCUGAGCAAc -3' miRNA: 3'- caGUGCCA--GUUAGUCUUA---GGCUUGUU- -5' |
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| 32259 | 3' | -48.2 | NC_007022.1 | + | 4196 | 0.69 | 0.994754 |
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Target: 5'- uUCugGGcUCGAUUu-GAUCCGAACAAa -3' miRNA: 3'- cAGugCC-AGUUAGucUUAGGCUUGUU- -5' |
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| 32259 | 3' | -48.2 | NC_007022.1 | + | 71473 | 1.07 | 0.023085 |
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Target: 5'- uGUCACGGUCAAUCAGAAUCCGAACAAa -3' miRNA: 3'- -CAGUGCCAGUUAGUCUUAGGCUUGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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