Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32260 | 3' | -45.8 | NC_007022.1 | + | 153643 | 0.66 | 0.999988 |
Target: 5'- -uUCGCuGAGGCuccaauauaaACUGGGUUAuucgguGUCu -3' miRNA: 3'- guAGCG-CUCCG----------UGACCUAAUuuu---UAG- -5' |
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32260 | 3' | -45.8 | NC_007022.1 | + | 60480 | 0.66 | 0.999988 |
Target: 5'- --aCGCGAGGCAaucGGAUuugccUGAAGAa- -3' miRNA: 3'- guaGCGCUCCGUga-CCUA-----AUUUUUag -5' |
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32260 | 3' | -45.8 | NC_007022.1 | + | 19139 | 0.66 | 0.999977 |
Target: 5'- cCAUCGCGAaucuGGUAUUGGAcgUUGuuugugcacauAAAGUCc -3' miRNA: 3'- -GUAGCGCU----CCGUGACCU--AAU-----------UUUUAG- -5' |
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32260 | 3' | -45.8 | NC_007022.1 | + | 109536 | 0.67 | 0.999943 |
Target: 5'- uGUUGaCGGGGCgaaACUGGAcuUUGAGAcUCg -3' miRNA: 3'- gUAGC-GCUCCG---UGACCU--AAUUUUuAG- -5' |
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32260 | 3' | -45.8 | NC_007022.1 | + | 168039 | 0.68 | 0.999868 |
Target: 5'- aCAUCccacgGCGGGGUcuCaGGGUUAGAAGUCu -3' miRNA: 3'- -GUAG-----CGCUCCGu-GaCCUAAUUUUUAG- -5' |
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32260 | 3' | -45.8 | NC_007022.1 | + | 133930 | 0.68 | 0.999639 |
Target: 5'- --cCGUagaGGGCAUUcGGAUUAAAAAUCg -3' miRNA: 3'- guaGCGc--UCCGUGA-CCUAAUUUUUAG- -5' |
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32260 | 3' | -45.8 | NC_007022.1 | + | 56580 | 0.68 | 0.999639 |
Target: 5'- cCGUCGCGGGuaucaauCGCUGGcUUAAGAAUa -3' miRNA: 3'- -GUAGCGCUCc------GUGACCuAAUUUUUAg -5' |
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32260 | 3' | -45.8 | NC_007022.1 | + | 8323 | 0.7 | 0.998915 |
Target: 5'- aUAUUGCGAGGCgggacaacaacGCUGGcugUAAAGGUg -3' miRNA: 3'- -GUAGCGCUCCG-----------UGACCua-AUUUUUAg -5' |
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32260 | 3' | -45.8 | NC_007022.1 | + | 104786 | 0.71 | 0.996057 |
Target: 5'- cUAUCGCGaAGGCaaACUGGcu--GAAAUCg -3' miRNA: 3'- -GUAGCGC-UCCG--UGACCuaauUUUUAG- -5' |
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32260 | 3' | -45.8 | NC_007022.1 | + | 123743 | 0.72 | 0.992637 |
Target: 5'- uUAUCGcCGAGGC-UUGGAU--AAAAUCa -3' miRNA: 3'- -GUAGC-GCUCCGuGACCUAauUUUUAG- -5' |
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32260 | 3' | -45.8 | NC_007022.1 | + | 74189 | 1.12 | 0.020941 |
Target: 5'- uCAUCGCGAGGCACUGGAUUAAAAAUCg -3' miRNA: 3'- -GUAGCGCUCCGUGACCUAAUUUUUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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