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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32260 | 5' | -43.9 | NC_007022.1 | + | 31654 | 0.66 | 1 |
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Target: 5'- cGGAUUUcUUGGuUUGCuauucgauUCCAACGg -3' miRNA: 3'- aCUUAGAaAACCuAACGu-------AGGUUGCa -5' |
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| 32260 | 5' | -43.9 | NC_007022.1 | + | 84379 | 0.67 | 0.999999 |
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Target: 5'- cUGggUCUuugagcccauaaUUUGGcgcagGCGUCCAugGa -3' miRNA: 3'- -ACuuAGA------------AAACCuaa--CGUAGGUugCa -5' |
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| 32260 | 5' | -43.9 | NC_007022.1 | + | 97213 | 0.67 | 0.999997 |
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Target: 5'- ---uUCUUgcUGGAaUGCAUCUAGCGc -3' miRNA: 3'- acuuAGAAa-ACCUaACGUAGGUUGCa -5' |
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| 32260 | 5' | -43.9 | NC_007022.1 | + | 88742 | 0.68 | 0.999993 |
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Target: 5'- aGAGUCaaagaacaucUGGAUUGUAUCCAGuCGc -3' miRNA: 3'- aCUUAGaaa-------ACCUAACGUAGGUU-GCa -5' |
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| 32260 | 5' | -43.9 | NC_007022.1 | + | 114916 | 0.69 | 0.999974 |
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Target: 5'- ---cUCUUUUGGuuUUGCgacaGUCCAACGa -3' miRNA: 3'- acuuAGAAAACCu-AACG----UAGGUUGCa -5' |
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| 32260 | 5' | -43.9 | NC_007022.1 | + | 154449 | 0.7 | 0.999848 |
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Target: 5'- cGAAUUuuaUUUUGGAcaggGCGUCCAACc- -3' miRNA: 3'- aCUUAG---AAAACCUaa--CGUAGGUUGca -5' |
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| 32260 | 5' | -43.9 | NC_007022.1 | + | 74227 | 1.09 | 0.049075 |
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Target: 5'- uUGAAUCUUUUGGAUUGCAUCCAACGUu -3' miRNA: 3'- -ACUUAGAAAACCUAACGUAGGUUGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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