Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32262 | 3' | -45 | NC_007022.1 | + | 166493 | 0.66 | 0.999994 |
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Target: 5'- uUGAUG--UACUCUGCuucuGGUgUGGUg- -3' miRNA: 3'- -ACUAUuaAUGAGGCGu---CCAaACCAau -5' |
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| 32262 | 3' | -45 | NC_007022.1 | + | 150981 | 0.66 | 0.999992 |
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Target: 5'- cGAUAAgccgACUCCGCAaGaGUUaGGUg- -3' miRNA: 3'- aCUAUUaa--UGAGGCGU-C-CAAaCCAau -5' |
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| 32262 | 3' | -45 | NC_007022.1 | + | 10373 | 0.68 | 0.999942 |
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Target: 5'- cGAUGuacACUuuGcCAGGUUUGGUc- -3' miRNA: 3'- aCUAUuaaUGAggC-GUCCAAACCAau -5' |
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| 32262 | 3' | -45 | NC_007022.1 | + | 113251 | 0.68 | 0.999896 |
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Target: 5'- cGcgAAUUACUCCGCA---UUGGUa- -3' miRNA: 3'- aCuaUUAAUGAGGCGUccaAACCAau -5' |
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| 32262 | 3' | -45 | NC_007022.1 | + | 109368 | 0.76 | 0.965791 |
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Target: 5'- gUGAU--UUACaaUCuCGCAGGUUUGGUUAu -3' miRNA: 3'- -ACUAuuAAUG--AG-GCGUCCAAACCAAU- -5' |
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| 32262 | 3' | -45 | NC_007022.1 | + | 109124 | 1.09 | 0.03417 |
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Target: 5'- aUGAUAAUUACUCCGCAGGUUUGGUUAg -3' miRNA: 3'- -ACUAUUAAUGAGGCGUCCAAACCAAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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