miRNA: 3'- -AGUGGaCCUGGUCCAuauccuuUGGU- -5'
miRNA: 3'- aGUGGACCuGGUCCAUau---------CCU-----UUGGU- -5'
miRNA: 3'- aGUGGaCCUGGUCca-UAUCCUuUGGu -5'
miRNA: 3'- -AGUGGACCUGGUCCAUAUCCUUUGgu -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32263 | 5' | -51.6 | NC_007022.1 | + | 57664 | 0.66 | 0.991786 |
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Target: 5'- aUCACC-GGGCCAGGUuuuu---GCCAu -3' miRNA: 3'- -AGUGGaCCUGGUCCAuauccuuUGGU- -5' |
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| 32263 | 5' | -51.6 | NC_007022.1 | + | 120924 | 0.68 | 0.96941 |
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Target: 5'- aUACCUGGuCCAGGUGaacuaguccguGGAgcuugGACCAg -3' miRNA: 3'- aGUGGACCuGGUCCAUau---------CCU-----UUGGU- -5' |
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| 32263 | 5' | -51.6 | NC_007022.1 | + | 62090 | 0.69 | 0.947903 |
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Target: 5'- aCACC-GGACCAGaucGUGGGAuGCCu -3' miRNA: 3'- aGUGGaCCUGGUCca-UAUCCUuUGGu -5' |
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| 32263 | 5' | -51.6 | NC_007022.1 | + | 120735 | 1.01 | 0.025439 |
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Target: 5'- uUCACCUGGACCAGGUAUAGGAAACa- -3' miRNA: 3'- -AGUGGACCUGGUCCAUAUCCUUUGgu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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