Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32267 | 3' | -42.3 | NC_007022.1 | + | 46129 | 0.66 | 1 |
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Target: 5'- --aUGAUCggUGUuggcgcugcaaugugUGCUGUAGguGAa -3' miRNA: 3'- uuaAUUAGuuGCA---------------ACGAUAUCguCUg -5' |
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| 32267 | 3' | -42.3 | NC_007022.1 | + | 108502 | 0.67 | 1 |
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Target: 5'- -uUUGGUCAuCGagGUUGUAGgAGACg -3' miRNA: 3'- uuAAUUAGUuGCaaCGAUAUCgUCUG- -5' |
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| 32267 | 3' | -42.3 | NC_007022.1 | + | 126720 | 0.67 | 1 |
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Target: 5'- --aUAAUUAAUaaUGCUAUGGaCGGGCa -3' miRNA: 3'- uuaAUUAGUUGcaACGAUAUC-GUCUG- -5' |
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| 32267 | 3' | -42.3 | NC_007022.1 | + | 26899 | 0.68 | 1 |
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Target: 5'- ------aCGACGcUGCUGUAGCuGAUc -3' miRNA: 3'- uuaauuaGUUGCaACGAUAUCGuCUG- -5' |
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| 32267 | 3' | -42.3 | NC_007022.1 | + | 115060 | 0.67 | 1 |
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Target: 5'- --cUGAUCAACGgUGauuucuUGUGGCAGAa -3' miRNA: 3'- uuaAUUAGUUGCaACg-----AUAUCGUCUg -5' |
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| 32267 | 3' | -42.3 | NC_007022.1 | + | 167966 | 0.69 | 0.999996 |
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Target: 5'- uAUUAAUCcACGUUGauaaccAGCAGGCg -3' miRNA: 3'- uUAAUUAGuUGCAACgaua--UCGUCUG- -5' |
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| 32267 | 3' | -42.3 | NC_007022.1 | + | 104773 | 0.72 | 0.999861 |
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Target: 5'- --cUGAUCAACGcUGCUAUcGCgaAGGCa -3' miRNA: 3'- uuaAUUAGUUGCaACGAUAuCG--UCUG- -5' |
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| 32267 | 3' | -42.3 | NC_007022.1 | + | 97472 | 0.74 | 0.997772 |
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Target: 5'- ---aAAUCAGgugcgacguggUGUUGCUGUGGUAGACc -3' miRNA: 3'- uuaaUUAGUU-----------GCAACGAUAUCGUCUG- -5' |
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| 32267 | 3' | -42.3 | NC_007022.1 | + | 144967 | 1.1 | 0.051647 |
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Target: 5'- aAAUUAAUCAACGUUGCUAUAGCAGACa -3' miRNA: 3'- -UUAAUUAGUUGCAACGAUAUCGUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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