miRNA: 3'- gACAGACUGCCGca---AUUGCAACuaaa -5'
miRNA: 3'- gaCAGa--------CUGCCGCaAUUGCAACUaaa -5'
miRNA: 3'- -GACAG-------ACUGCCGCAAUUgcAACUAaa -5'
miRNA: 3'- -GACAGACUGCCGCAAUUGCAACUAAA- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32267 | 5' | -46.9 | NC_007022.1 | + | 151099 | 0.66 | 0.999896 |
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Target: 5'- uUGUUUGACGGCucuggUGAUGUUGu--- -3' miRNA: 3'- gACAGACUGCCGca---AUUGCAACuaaa -5' |
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| 32267 | 5' | -46.9 | NC_007022.1 | + | 81289 | 0.68 | 0.99942 |
|
Target: 5'- -cGUCggcacacagGGCGGCGgUAAUGUUGAa-- -3' miRNA: 3'- gaCAGa--------CUGCCGCaAUUGCAACUaaa -5' |
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| 32267 | 5' | -46.9 | NC_007022.1 | + | 118758 | 0.7 | 0.994526 |
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Target: 5'- aCUGUCaauugguUGGCGGCGUUGAgaUUGAUa- -3' miRNA: 3'- -GACAG-------ACUGCCGCAAUUgcAACUAaa -5' |
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| 32267 | 5' | -46.9 | NC_007022.1 | + | 145002 | 1.06 | 0.036507 |
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Target: 5'- cCUGUCUGACGGCGUUAACGUUGAUUUc -3' miRNA: 3'- -GACAGACUGCCGCAAUUGCAACUAAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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