Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32272 | 3' | -53.2 | NC_007023.1 | + | 121017 | 0.66 | 0.975382 |
Target: 5'- ---aUGCUGGUAUGUAccaaaaccGCGACCUg -3' miRNA: 3'- uuucACGACCGUGCAUuac-----CGCUGGG- -5' |
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32272 | 3' | -53.2 | NC_007023.1 | + | 143630 | 0.66 | 0.975122 |
Target: 5'- uAAGcGCUGGUGauguuguUGUAAcGGCGACCUc -3' miRNA: 3'- uUUCaCGACCGU-------GCAUUaCCGCUGGG- -5' |
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32272 | 3' | -53.2 | NC_007023.1 | + | 172354 | 0.66 | 0.971849 |
Target: 5'- ---cUGCUGGUAcuacaucauaccuuCGUAaauuccgagGUGGUGGCCCu -3' miRNA: 3'- uuucACGACCGU--------------GCAU---------UACCGCUGGG- -5' |
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32272 | 3' | -53.2 | NC_007023.1 | + | 146857 | 0.67 | 0.952033 |
Target: 5'- gGAAGgGCU-GCACGgucaaucGGUGGUGAUCCg -3' miRNA: 3'- -UUUCaCGAcCGUGCa------UUACCGCUGGG- -5' |
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32272 | 3' | -53.2 | NC_007023.1 | + | 100629 | 0.67 | 0.952033 |
Target: 5'- -uGGUGCUGGUuacuggaucAUGUucGGUGGUGGCUUg -3' miRNA: 3'- uuUCACGACCG---------UGCA--UUACCGCUGGG- -5' |
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32272 | 3' | -53.2 | NC_007023.1 | + | 16187 | 1.1 | 0.004985 |
Target: 5'- aAAAGUGCUGGCACGUAAUGGCGACCCu -3' miRNA: 3'- -UUUCACGACCGUGCAUUACCGCUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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