miRNA: 3'- gACCUAGa--UGUCC--UAGUACGugUCGU- -5'
miRNA: 3'- -GACCUagaUGUCC--UAGUACGUguCGU- -5'
miRNA: 3'- -GACCUAgaugUCCUAGUAcGUGUCGU- -5'
miRNA: 3'- gaCCUAGaUGUCCUa---GUa---CGUGUCGU- -5'
miRNA: 3'- -GACCUAGAUGUCCUAGUACGUGUCGU- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32272 | 5' | -51 | NC_007023.1 | + | 35527 | 0.66 | 0.993124 |
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Target: 5'- uUGGAUUcaaACAGGuaAUCGUGUugGGUAc -3' miRNA: 3'- gACCUAGa--UGUCC--UAGUACGugUCGU- -5' |
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| 32272 | 5' | -51 | NC_007023.1 | + | 90290 | 0.66 | 0.993124 |
|
Target: 5'- aCUGGA---ACAGGcgAUCAUGUAguGCGu -3' miRNA: 3'- -GACCUagaUGUCC--UAGUACGUguCGU- -5' |
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| 32272 | 5' | -51 | NC_007023.1 | + | 106740 | 0.72 | 0.876872 |
|
Target: 5'- cCUGGAUa---GGGAUCAUcCACGGCAa -3' miRNA: 3'- -GACCUAgaugUCCUAGUAcGUGUCGU- -5' |
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| 32272 | 5' | -51 | NC_007023.1 | + | 97764 | 0.74 | 0.791814 |
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Target: 5'- -aGGAUCaGCAGGAguagCAacgcGCACAGCAu -3' miRNA: 3'- gaCCUAGaUGUCCUa---GUa---CGUGUCGU- -5' |
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| 32272 | 5' | -51 | NC_007023.1 | + | 16148 | 1.1 | 0.008168 |
|
Target: 5'- gCUGGAUCUACAGGAUCAUGCACAGCAa -3' miRNA: 3'- -GACCUAGAUGUCCUAGUACGUGUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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