Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32276 | 3' | -52.5 | NC_007023.1 | + | 155667 | 0.66 | 0.986617 |
Target: 5'- gACGAUAUUCGauguaugcaCGGGCGAcCGCuGGGUu -3' miRNA: 3'- -UGUUAUAGGUa--------GUCCGCUaGCG-CCCG- -5' |
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32276 | 3' | -52.5 | NC_007023.1 | + | 14664 | 0.66 | 0.983049 |
Target: 5'- cCGAUGUCgGUCGGG-GAUa-UGGGCg -3' miRNA: 3'- uGUUAUAGgUAGUCCgCUAgcGCCCG- -5' |
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32276 | 3' | -52.5 | NC_007023.1 | + | 124026 | 0.67 | 0.981016 |
Target: 5'- uACGAUAaCCcgUAuaacGCGGUUGUGGGCg -3' miRNA: 3'- -UGUUAUaGGuaGUc---CGCUAGCGCCCG- -5' |
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32276 | 3' | -52.5 | NC_007023.1 | + | 172692 | 0.67 | 0.973822 |
Target: 5'- cACAAUAcCCcguAUCAGGUacaGAUCGCGGu- -3' miRNA: 3'- -UGUUAUaGG---UAGUCCG---CUAGCGCCcg -5' |
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32276 | 3' | -52.5 | NC_007023.1 | + | 65754 | 0.68 | 0.968038 |
Target: 5'- aGCAAUGgucgcaaacauUCCgGUCGGGUGuUCGUGuGGCu -3' miRNA: 3'- -UGUUAU-----------AGG-UAGUCCGCuAGCGC-CCG- -5' |
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32276 | 3' | -52.5 | NC_007023.1 | + | 108216 | 0.68 | 0.94975 |
Target: 5'- uCAAUAUCCAUCAuuGCGuuuuUCGCGGu- -3' miRNA: 3'- uGUUAUAGGUAGUc-CGCu---AGCGCCcg -5' |
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32276 | 3' | -52.5 | NC_007023.1 | + | 84752 | 0.72 | 0.826394 |
Target: 5'- uGCuGUAUgUUAUCAGGUGAUCGCGGa- -3' miRNA: 3'- -UGuUAUA-GGUAGUCCGCUAGCGCCcg -5' |
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32276 | 3' | -52.5 | NC_007023.1 | + | 56555 | 1.12 | 0.004976 |
Target: 5'- cACAAUAUCCAUCAGGCGAUCGCGGGCu -3' miRNA: 3'- -UGUUAUAGGUAGUCCGCUAGCGCCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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