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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
32277 | 5' | -50.1 | NC_007023.1 | + | 109454 | 0.66 | 0.996161 |
Target: 5'- aGCAUca-AUGAUCGCCUGgaaugaaucUUUCAGUg -3' miRNA: 3'- -CGUGaugUACUAGCGGAC---------AAGGUCAa -5' |
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32277 | 5' | -50.1 | NC_007023.1 | + | 15943 | 0.68 | 0.984401 |
Target: 5'- uGCugUGCAUGAU--CCUGUagaUCCAGa- -3' miRNA: 3'- -CGugAUGUACUAgcGGACA---AGGUCaa -5' |
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32277 | 5' | -50.1 | NC_007023.1 | + | 90495 | 1.09 | 0.010746 |
Target: 5'- cGCACUACAUGAUCGCCUGUUCCAGUUa -3' miRNA: 3'- -CGUGAUGUACUAGCGGACAAGGUCAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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