Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32278 | 3' | -45.8 | NC_007023.1 | + | 37888 | 0.66 | 0.999995 |
Target: 5'- aGGUGCGuauguGUUUAgUGCgGUAuacGACCCAg -3' miRNA: 3'- -CCAUGUu----UAGGUaACGgCAU---UUGGGU- -5' |
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32278 | 3' | -45.8 | NC_007023.1 | + | 3286 | 0.66 | 0.999993 |
Target: 5'- aGGUAuCGAGUCUGgaaGCCGUAGAgCUu -3' miRNA: 3'- -CCAU-GUUUAGGUaa-CGGCAUUUgGGu -5' |
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32278 | 3' | -45.8 | NC_007023.1 | + | 98737 | 0.66 | 0.999993 |
Target: 5'- -cUGCAA--CCcgUGCCGcUGAACCUAu -3' miRNA: 3'- ccAUGUUuaGGuaACGGC-AUUUGGGU- -5' |
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32278 | 3' | -45.8 | NC_007023.1 | + | 39948 | 0.66 | 0.99999 |
Target: 5'- -cUGCAAAUCCucuUUGCaGUGucCCCAc -3' miRNA: 3'- ccAUGUUUAGGu--AACGgCAUuuGGGU- -5' |
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32278 | 3' | -45.8 | NC_007023.1 | + | 49995 | 0.67 | 0.999952 |
Target: 5'- gGGUAaacuUAAAUCCcgUGCCGaUGAAgUCAu -3' miRNA: 3'- -CCAU----GUUUAGGuaACGGC-AUUUgGGU- -5' |
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32278 | 3' | -45.8 | NC_007023.1 | + | 46214 | 0.68 | 0.999811 |
Target: 5'- uGGUGCGAcguauUCCAUguUGaCgGUGAAUCCAu -3' miRNA: 3'- -CCAUGUUu----AGGUA--AC-GgCAUUUGGGU- -5' |
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32278 | 3' | -45.8 | NC_007023.1 | + | 147767 | 0.71 | 0.997961 |
Target: 5'- --aACGAAUCCGcgcuuuccgUGCCGaAAACCCGa -3' miRNA: 3'- ccaUGUUUAGGUa--------ACGGCaUUUGGGU- -5' |
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32278 | 3' | -45.8 | NC_007023.1 | + | 96487 | 0.71 | 0.997558 |
Target: 5'- cGUGgAAGUUCAUacuuUGCUGUAAACCUg -3' miRNA: 3'- cCAUgUUUAGGUA----ACGGCAUUUGGGu -5' |
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32278 | 3' | -45.8 | NC_007023.1 | + | 107021 | 0.72 | 0.996552 |
Target: 5'- aGGUACAGAUCCAgcaGuuGUuAACgCAg -3' miRNA: 3'- -CCAUGUUUAGGUaa-CggCAuUUGgGU- -5' |
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32278 | 3' | -45.8 | NC_007023.1 | + | 149559 | 0.72 | 0.993512 |
Target: 5'- cGGcUACccuGAUCgGUaGCCGUGAACCCc -3' miRNA: 3'- -CC-AUGu--UUAGgUAaCGGCAUUUGGGu -5' |
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32278 | 3' | -45.8 | NC_007023.1 | + | 109788 | 1.13 | 0.020453 |
Target: 5'- uGGUACAAAUCCAUUGCCGUAAACCCAg -3' miRNA: 3'- -CCAUGUUUAGGUAACGGCAUUUGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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