Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32280 | 5' | -50 | NC_007023.1 | + | 44319 | 0.66 | 0.9978 |
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Target: 5'- -aAUGGGCUGAACaCUuauUCAGUauuGGUg -3' miRNA: 3'- uaUGCCCGAUUUG-GAuauAGUCG---CCA- -5' |
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| 32280 | 5' | -50 | NC_007023.1 | + | 140746 | 0.66 | 0.9978 |
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Target: 5'- -aACGcGCUAaucuauguuucAACCUGgcaAUCAGCGGUu -3' miRNA: 3'- uaUGCcCGAU-----------UUGGAUa--UAGUCGCCA- -5' |
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| 32280 | 5' | -50 | NC_007023.1 | + | 136213 | 0.66 | 0.997377 |
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Target: 5'- cUACGGGCUucgauAACCUGUuuGUCGauaCGGa -3' miRNA: 3'- uAUGCCCGAu----UUGGAUA--UAGUc--GCCa -5' |
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| 32280 | 5' | -50 | NC_007023.1 | + | 33108 | 0.68 | 0.989661 |
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Target: 5'- -gACGGGCUAAACC---AUgAGCGa- -3' miRNA: 3'- uaUGCCCGAUUUGGauaUAgUCGCca -5' |
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| 32280 | 5' | -50 | NC_007023.1 | + | 170702 | 0.7 | 0.963762 |
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Target: 5'- ---gGGGCUAAACCUggugAUGUCAuccguauuGCGGg -3' miRNA: 3'- uaugCCCGAUUUGGA----UAUAGU--------CGCCa -5' |
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| 32280 | 5' | -50 | NC_007023.1 | + | 147030 | 1.07 | 0.014979 |
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Target: 5'- gAUACGGGCUAAACCUAUAUCAGCGGUg -3' miRNA: 3'- -UAUGCCCGAUUUGGAUAUAGUCGCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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