Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32281 | 3' | -51.5 | NC_007023.1 | + | 92161 | 0.66 | 0.985521 |
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Target: 5'- gAUAGCAUCGCguUCugacauuGCCAUUAUCg -3' miRNA: 3'- -UAUUGUGGCGguAGuau----CGGUGGUAG- -5' |
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| 32281 | 3' | -51.5 | NC_007023.1 | + | 121093 | 0.66 | 0.985343 |
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Target: 5'- --cACACUGCCAUgAUGGuaaguuaCCACCAg- -3' miRNA: 3'- uauUGUGGCGGUAgUAUC-------GGUGGUag -5' |
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| 32281 | 3' | -51.5 | NC_007023.1 | + | 1119 | 0.67 | 0.981625 |
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Target: 5'- cGUuuCACCGUCuUCuGUAGUCugCAUCa -3' miRNA: 3'- -UAuuGUGGCGGuAG-UAUCGGugGUAG- -5' |
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| 32281 | 3' | -51.5 | NC_007023.1 | + | 76032 | 0.68 | 0.958012 |
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Target: 5'- --uACAUgucaCGaCCAUCAUAGCCGCCcgUg -3' miRNA: 3'- uauUGUG----GC-GGUAGUAUCGGUGGuaG- -5' |
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| 32281 | 3' | -51.5 | NC_007023.1 | + | 66722 | 0.69 | 0.940611 |
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Target: 5'- aGUAACAgUGCCAUgAU-GCCGCCGc- -3' miRNA: 3'- -UAUUGUgGCGGUAgUAuCGGUGGUag -5' |
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| 32281 | 3' | -51.5 | NC_007023.1 | + | 11994 | 0.69 | 0.930385 |
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Target: 5'- -aAGCACCGCgCAUCAaccguGCgCugCAUCg -3' miRNA: 3'- uaUUGUGGCG-GUAGUau---CG-GugGUAG- -5' |
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| 32281 | 3' | -51.5 | NC_007023.1 | + | 166385 | 0.7 | 0.919125 |
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Target: 5'- uUGAUACCaaCCAUCcc-GCCGCCAUCu -3' miRNA: 3'- uAUUGUGGc-GGUAGuauCGGUGGUAG- -5' |
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| 32281 | 3' | -51.5 | NC_007023.1 | + | 116586 | 0.83 | 0.310664 |
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Target: 5'- uUAACACCGCCGUCAUacagcgGGUCACCAg- -3' miRNA: 3'- uAUUGUGGCGGUAGUA------UCGGUGGUag -5' |
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| 32281 | 3' | -51.5 | NC_007023.1 | + | 151237 | 1.08 | 0.00859 |
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Target: 5'- cAUAACACCGCCAUCAUAGCCACCAUCu -3' miRNA: 3'- -UAUUGUGGCGGUAGUAUCGGUGGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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