Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32283 | 3' | -42.6 | NC_007023.1 | + | 179692 | 0.68 | 1 |
Target: 5'- cACCAGUC------UUGCGGCGUUgauucggUGCu -3' miRNA: 3'- -UGGUUAGcuauaaAAUGCCGCAA-------ACG- -5' |
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32283 | 3' | -42.6 | NC_007023.1 | + | 170436 | 0.67 | 1 |
Target: 5'- -gCAAUUGGUGUga-ACGGUGUgcguaUGCa -3' miRNA: 3'- ugGUUAGCUAUAaaaUGCCGCAa----ACG- -5' |
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32283 | 3' | -42.6 | NC_007023.1 | + | 165187 | 0.66 | 1 |
Target: 5'- uACCGAUCGAaAUUUaGCGaCGg--GCa -3' miRNA: 3'- -UGGUUAGCUaUAAAaUGCcGCaaaCG- -5' |
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32283 | 3' | -42.6 | NC_007023.1 | + | 89432 | 0.67 | 1 |
Target: 5'- -aCAA-CGGgcg-UUGCGGCGUUUGa -3' miRNA: 3'- ugGUUaGCUauaaAAUGCCGCAAACg -5' |
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32283 | 3' | -42.6 | NC_007023.1 | + | 178263 | 0.67 | 1 |
Target: 5'- uGCCAAUCGGUAagcguaaaGGCGaagugaUUGCa -3' miRNA: 3'- -UGGUUAGCUAUaaaaug--CCGCa-----AACG- -5' |
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32283 | 3' | -42.6 | NC_007023.1 | + | 167601 | 0.67 | 1 |
Target: 5'- cGCguAUCGAgcuUUUUA-GGCGUgaUUGCg -3' miRNA: 3'- -UGguUAGCUau-AAAAUgCCGCA--AACG- -5' |
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32283 | 3' | -42.6 | NC_007023.1 | + | 96461 | 0.69 | 0.999999 |
Target: 5'- -gUAAUCGGUucg-UGCGGC-UUUGCg -3' miRNA: 3'- ugGUUAGCUAuaaaAUGCCGcAAACG- -5' |
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32283 | 3' | -42.6 | NC_007023.1 | + | 79416 | 0.69 | 0.999998 |
Target: 5'- cACCGAUCGAgaaau--CGGCGUgucGUa -3' miRNA: 3'- -UGGUUAGCUauaaaauGCCGCAaa-CG- -5' |
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32283 | 3' | -42.6 | NC_007023.1 | + | 113435 | 0.71 | 0.999987 |
Target: 5'- aACUcgGAUCGGUGUcgauCGGUGUUUGUu -3' miRNA: 3'- -UGG--UUAGCUAUAaaauGCCGCAAACG- -5' |
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32283 | 3' | -42.6 | NC_007023.1 | + | 7633 | 0.72 | 0.999955 |
Target: 5'- aACgGAUCGGgg--UUGCGGC-UUUGCu -3' miRNA: 3'- -UGgUUAGCUauaaAAUGCCGcAAACG- -5' |
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32283 | 3' | -42.6 | NC_007023.1 | + | 132558 | 0.72 | 0.999892 |
Target: 5'- gACCuacGUCGAUAUagaacacACGGCGUUcaggUGCg -3' miRNA: 3'- -UGGu--UAGCUAUAaaa----UGCCGCAA----ACG- -5' |
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32283 | 3' | -42.6 | NC_007023.1 | + | 169697 | 1.15 | 0.038902 |
Target: 5'- aACCAAUCGAUAUUUUACGGCGUUUGCu -3' miRNA: 3'- -UGGUUAGCUAUAAAAUGCCGCAAACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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