miRNA display CGI


Results 1 - 11 of 11 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
32283 5' -48.3 NC_007023.1 + 157271 0.66 0.999822
Target:  5'- -aGCGUUacaCGCCGUAcaugcGGUUAUCuGGGu -3'
miRNA:   3'- ugUGUAA---GCGGCAUu----UCGAUAGuCCC- -5'
32283 5' -48.3 NC_007023.1 + 10687 0.66 0.999634
Target:  5'- uUACAUUC-CCGaAAuGgUAUCAGGGa -3'
miRNA:   3'- uGUGUAAGcGGCaUUuCgAUAGUCCC- -5'
32283 5' -48.3 NC_007023.1 + 117916 0.67 0.998704
Target:  5'- aGCACGgaCGuuGUAcGAGUUAcCAGGGc -3'
miRNA:   3'- -UGUGUaaGCggCAU-UUCGAUaGUCCC- -5'
32283 5' -48.3 NC_007023.1 + 49000 0.68 0.998433
Target:  5'- uCAUAUgauccgCGCCaUAAAGCaaaGUCAGGGu -3'
miRNA:   3'- uGUGUAa-----GCGGcAUUUCGa--UAGUCCC- -5'
32283 5' -48.3 NC_007023.1 + 149355 0.68 0.997744
Target:  5'- aACACGUUCaCCGUucGGCaugaaagCAGGGu -3'
miRNA:   3'- -UGUGUAAGcGGCAuuUCGaua----GUCCC- -5'
32283 5' -48.3 NC_007023.1 + 178512 0.68 0.997744
Target:  5'- uACACucUCG-UGUAGAGC-GUCGGGGa -3'
miRNA:   3'- -UGUGuaAGCgGCAUUUCGaUAGUCCC- -5'
32283 5' -48.3 NC_007023.1 + 16291 0.68 0.996819
Target:  5'- -uGCAUUguuuucCGCUGUAcuugcaggacgcAGGUUAUCAGGGa -3'
miRNA:   3'- ugUGUAA------GCGGCAU------------UUCGAUAGUCCC- -5'
32283 5' -48.3 NC_007023.1 + 79328 0.68 0.996819
Target:  5'- -uGCAUUCG-UGUAGGGCUuuuguuauAUUAGGGg -3'
miRNA:   3'- ugUGUAAGCgGCAUUUCGA--------UAGUCCC- -5'
32283 5' -48.3 NC_007023.1 + 72788 0.69 0.996251
Target:  5'- aACACGUUCaCCGUGAcuuaGGCgGUCAcauGGGu -3'
miRNA:   3'- -UGUGUAAGcGGCAUU----UCGaUAGU---CCC- -5'
32283 5' -48.3 NC_007023.1 + 55536 0.75 0.882807
Target:  5'- gACGgGaUCGCCGUAAaauGGCaGUCGGGGu -3'
miRNA:   3'- -UGUgUaAGCGGCAUU---UCGaUAGUCCC- -5'
32283 5' -48.3 NC_007023.1 + 169663 1.11 0.015323
Target:  5'- aACACAUUCGCCGUAAAGCUAUCAGGGc -3'
miRNA:   3'- -UGUGUAAGCGGCAUUUCGAUAGUCCC- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.