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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
32284 | 3' | -53.5 | NC_007023.1 | + | 170429 | 0.66 | 0.960275 |
Target: 5'- -cGGUGCugCAAUUggugUGAACGGUGUg- -3' miRNA: 3'- uuCCGCGugGUUAA----GCUUGUCGCGau -5' |
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32284 | 3' | -53.5 | NC_007023.1 | + | 27477 | 0.67 | 0.933784 |
Target: 5'- cAGGCGCAUacuUAAUcgcuUCGGaucGCAGCGCUu -3' miRNA: 3'- uUCCGCGUG---GUUA----AGCU---UGUCGCGAu -5' |
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32284 | 3' | -53.5 | NC_007023.1 | + | 112235 | 0.68 | 0.898208 |
Target: 5'- -uGGUuuuGCAgUCAGUUCGAuauauGCAGCGCUAa -3' miRNA: 3'- uuCCG---CGU-GGUUAAGCU-----UGUCGCGAU- -5' |
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32284 | 3' | -53.5 | NC_007023.1 | + | 89428 | 0.71 | 0.792475 |
Target: 5'- cGGGCGUugCGgcGUUUGAAaCGGCGCUu -3' miRNA: 3'- uUCCGCGugGU--UAAGCUU-GUCGCGAu -5' |
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32284 | 3' | -53.5 | NC_007023.1 | + | 176138 | 1.04 | 0.008871 |
Target: 5'- aAAGGCGCACCAAUUCGAACAGCGCUAc -3' miRNA: 3'- -UUCCGCGUGGUUAAGCUUGUCGCGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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