Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32286 | 3' | -46.3 | NC_007023.1 | + | 47022 | 0.66 | 0.999979 |
Target: 5'- gGCGCUCUguugguugGGUGUGCugAAAAGCCCC-Ca -3' miRNA: 3'- -UGUGGGGa-------UUAUAUG--UUUUUGGGGaG- -5' |
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32286 | 3' | -46.3 | NC_007023.1 | + | 149241 | 0.66 | 0.999979 |
Target: 5'- -aACCCCguuAUGUGCAGGAAUCgCg- -3' miRNA: 3'- ugUGGGGau-UAUAUGUUUUUGGgGag -5' |
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32286 | 3' | -46.3 | NC_007023.1 | + | 158873 | 0.66 | 0.999979 |
Target: 5'- uGCAgCCa--GUAUACGAAcGCCCCg- -3' miRNA: 3'- -UGUgGGgauUAUAUGUUUuUGGGGag -5' |
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32286 | 3' | -46.3 | NC_007023.1 | + | 150148 | 0.66 | 0.999961 |
Target: 5'- cCGCCCUUGAc--ACAAAAAUCCUUUu -3' miRNA: 3'- uGUGGGGAUUauaUGUUUUUGGGGAG- -5' |
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32286 | 3' | -46.3 | NC_007023.1 | + | 125054 | 0.68 | 0.999564 |
Target: 5'- cACugCCCUAA-AUACAaauagggGAAACCCUa- -3' miRNA: 3'- -UGugGGGAUUaUAUGU-------UUUUGGGGag -5' |
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32286 | 3' | -46.3 | NC_007023.1 | + | 19418 | 0.68 | 0.999466 |
Target: 5'- aGCACCgaUGAUAaGCAuAAGCCCCa- -3' miRNA: 3'- -UGUGGggAUUAUaUGUuUUUGGGGag -5' |
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32286 | 3' | -46.3 | NC_007023.1 | + | 13741 | 0.69 | 0.998982 |
Target: 5'- -aACCUCaAAgggGCAAAAGCCCCUa -3' miRNA: 3'- ugUGGGGaUUauaUGUUUUUGGGGAg -5' |
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32286 | 3' | -46.3 | NC_007023.1 | + | 153092 | 0.71 | 0.995616 |
Target: 5'- cAUACCCaaCUAAcuUACAAAAaaGCCCCUUg -3' miRNA: 3'- -UGUGGG--GAUUauAUGUUUU--UGGGGAG- -5' |
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32286 | 3' | -46.3 | NC_007023.1 | + | 157603 | 0.72 | 0.991935 |
Target: 5'- gUAUgUgUAAUAUaACAAAAGCCCCUCa -3' miRNA: 3'- uGUGgGgAUUAUA-UGUUUUUGGGGAG- -5' |
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32286 | 3' | -46.3 | NC_007023.1 | + | 125281 | 0.73 | 0.980025 |
Target: 5'- uACACCCCaacAUAUAUAGGGuuuCCCCUa -3' miRNA: 3'- -UGUGGGGau-UAUAUGUUUUu--GGGGAg -5' |
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32286 | 3' | -46.3 | NC_007023.1 | + | 75673 | 0.76 | 0.940409 |
Target: 5'- aACACCUCUGAUuuuuUGCGA--ACCCCUg -3' miRNA: 3'- -UGUGGGGAUUAu---AUGUUuuUGGGGAg -5' |
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32286 | 3' | -46.3 | NC_007023.1 | + | 79113 | 0.84 | 0.597336 |
Target: 5'- gUACCCCUAAUAUaACAAAAGCCCUa- -3' miRNA: 3'- uGUGGGGAUUAUA-UGUUUUUGGGGag -5' |
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32286 | 3' | -46.3 | NC_007023.1 | + | 100769 | 0.87 | 0.422079 |
Target: 5'- aACACCCCcAAUAUaGCAAAAGCCCCUg -3' miRNA: 3'- -UGUGGGGaUUAUA-UGUUUUUGGGGAg -5' |
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32286 | 3' | -46.3 | NC_007023.1 | + | 67244 | 1.12 | 0.018777 |
Target: 5'- aACACCCCUAAUAUACAAAAACCCCUCg -3' miRNA: 3'- -UGUGGGGAUUAUAUGUUUUUGGGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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