Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32287 | 3' | -52.9 | NC_007023.1 | + | 120239 | 0.67 | 0.979389 |
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Target: 5'- gUCCgCGCGGGUacuauUCGCgACGGUUucGCa -3' miRNA: 3'- -AGG-GCGCUCAgu---AGCG-UGCCAAu-UGg -5' |
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| 32287 | 3' | -52.9 | NC_007023.1 | + | 23526 | 0.68 | 0.969008 |
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Target: 5'- aUCCCGCGAGUaa--G-ACGGUUGAa- -3' miRNA: 3'- -AGGGCGCUCAguagCgUGCCAAUUgg -5' |
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| 32287 | 3' | -52.9 | NC_007023.1 | + | 132643 | 0.68 | 0.965596 |
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Target: 5'- aCCCguGCGAG-CAUCGUacacaacGCGGUUuuuaAGCCc -3' miRNA: 3'- aGGG--CGCUCaGUAGCG-------UGCCAA----UUGG- -5' |
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| 32287 | 3' | -52.9 | NC_007023.1 | + | 43077 | 0.69 | 0.947194 |
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Target: 5'- --aCGCGAGuUCAUC-CACGGUUuugcaguACCa -3' miRNA: 3'- aggGCGCUC-AGUAGcGUGCCAAu------UGG- -5' |
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| 32287 | 3' | -52.9 | NC_007023.1 | + | 86056 | 0.77 | 0.569831 |
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Target: 5'- gCCCGUGAauaugC-UCGCAUGGUUAACCa -3' miRNA: 3'- aGGGCGCUca---GuAGCGUGCCAAUUGG- -5' |
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| 32287 | 3' | -52.9 | NC_007023.1 | + | 70313 | 1.14 | 0.003864 |
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Target: 5'- aUCCCGCGAGUCAUCGCACGGUUAACCa -3' miRNA: 3'- -AGGGCGCUCAGUAGCGUGCCAAUUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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