miRNA display CGI


Results 1 - 15 of 15 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
32288 3' -46.8 NC_007023.1 + 42017 0.66 0.999972
Target:  5'- aGUgaAUCACCUGaUUCAAUCAcuacgcaACUGg- -3'
miRNA:   3'- gCG--UAGUGGACaAAGUUAGU-------UGACgg -5'
32288 3' -46.8 NC_007023.1 + 55986 0.66 0.999934
Target:  5'- aGUGUCAUaacGUUaUCAGUCAGUUGCCg -3'
miRNA:   3'- gCGUAGUGga-CAA-AGUUAGUUGACGG- -5'
32288 3' -46.8 NC_007023.1 + 88105 0.67 0.999886
Target:  5'- aCGCAUCGCgagguugaaUUGUUUUcAUCAGCUuGCg -3'
miRNA:   3'- -GCGUAGUG---------GACAAAGuUAGUUGA-CGg -5'
32288 3' -46.8 NC_007023.1 + 33051 0.67 0.999852
Target:  5'- uGCgAUCACCaGUUUCAcggAUCuGCUGUUu -3'
miRNA:   3'- gCG-UAGUGGaCAAAGU---UAGuUGACGG- -5'
32288 3' -46.8 NC_007023.1 + 64337 0.67 0.999808
Target:  5'- aGUGuUUACCUGUUaaGGUCAGCaGCCc -3'
miRNA:   3'- gCGU-AGUGGACAAagUUAGUUGaCGG- -5'
32288 3' -46.8 NC_007023.1 + 83972 0.67 0.999808
Target:  5'- gGCGUguCCUGU-----UCAAUUGCCu -3'
miRNA:   3'- gCGUAguGGACAaaguuAGUUGACGG- -5'
32288 3' -46.8 NC_007023.1 + 170139 0.67 0.999808
Target:  5'- uCGUAUCAuCCUGU---GAUauACUGCCa -3'
miRNA:   3'- -GCGUAGU-GGACAaagUUAguUGACGG- -5'
32288 3' -46.8 NC_007023.1 + 89538 0.67 0.999804
Target:  5'- aGCAUCGCCUGUaauguguuuugaaUUCAugGUUuguGACgcGCCa -3'
miRNA:   3'- gCGUAGUGGACA-------------AAGU--UAG---UUGa-CGG- -5'
32288 3' -46.8 NC_007023.1 + 115257 0.68 0.999688
Target:  5'- aUGCuUCACCUGUUaCAagGUCAAaUGUCu -3'
miRNA:   3'- -GCGuAGUGGACAAaGU--UAGUUgACGG- -5'
32288 3' -46.8 NC_007023.1 + 46577 0.69 0.999236
Target:  5'- uGUAUCAUCcaggaauaauUGUUUCAggucAUCAACUGUg -3'
miRNA:   3'- gCGUAGUGG----------ACAAAGU----UAGUUGACGg -5'
32288 3' -46.8 NC_007023.1 + 118328 0.7 0.996785
Target:  5'- cCGCGUCACCUGcUUUAAcaaguuggcgaccguUCAACaGCa -3'
miRNA:   3'- -GCGUAGUGGACaAAGUU---------------AGUUGaCGg -5'
32288 3' -46.8 NC_007023.1 + 90496 0.7 0.996562
Target:  5'- aCGCAcuacaugaUCGCCUGUUcCAGUUAACUuuuCCa -3'
miRNA:   3'- -GCGU--------AGUGGACAAaGUUAGUUGAc--GG- -5'
32288 3' -46.8 NC_007023.1 + 104066 0.7 0.996562
Target:  5'- aGCAUUACCacgGUcaaccaGAUCAACUGUCu -3'
miRNA:   3'- gCGUAGUGGa--CAaag---UUAGUUGACGG- -5'
32288 3' -46.8 NC_007023.1 + 107966 0.72 0.988713
Target:  5'- aCGCGUCACCU---UCAAUUAccacuuguaaAUUGCCu -3'
miRNA:   3'- -GCGUAGUGGAcaaAGUUAGU----------UGACGG- -5'
32288 3' -46.8 NC_007023.1 + 78818 1.09 0.028174
Target:  5'- aCGCAUCACCUGUUUCAAUCAACUGCa -3'
miRNA:   3'- -GCGUAGUGGACAAAGUUAGUUGACGg -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.