Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32289 | 3' | -57.3 | NC_007023.1 | + | 152140 | 0.66 | 0.883761 |
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Target: 5'- aGCAga-GGUCGCgCCCC-GC--GCCCCa -3' miRNA: 3'- -CGUaggUUAGCG-GGGGuCGuuCGGGG- -5' |
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| 32289 | 3' | -57.3 | NC_007023.1 | + | 47645 | 0.66 | 0.876819 |
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Target: 5'- gGUAUCCugugaGAUCGCCgCCAGCAcaguaaagGGUUCg -3' miRNA: 3'- -CGUAGG-----UUAGCGGgGGUCGU--------UCGGGg -5' |
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| 32289 | 3' | -57.3 | NC_007023.1 | + | 169509 | 0.66 | 0.869667 |
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Target: 5'- cGUGUCUAcuGUCGaa-CCAGCAaccgcaacAGCCCCg -3' miRNA: 3'- -CGUAGGU--UAGCgggGGUCGU--------UCGGGG- -5' |
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| 32289 | 3' | -57.3 | NC_007023.1 | + | 74778 | 0.7 | 0.682727 |
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Target: 5'- uGUAUCCu-UCgGCCUCCAG-AAGCCCg -3' miRNA: 3'- -CGUAGGuuAG-CGGGGGUCgUUCGGGg -5' |
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| 32289 | 3' | -57.3 | NC_007023.1 | + | 167686 | 0.7 | 0.682727 |
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Target: 5'- aGCAUCCccacGUaaaGCCCCCucAGCAgucguAGCaCCCg -3' miRNA: 3'- -CGUAGGu---UAg--CGGGGG--UCGU-----UCG-GGG- -5' |
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| 32289 | 3' | -57.3 | NC_007023.1 | + | 19400 | 0.7 | 0.666758 |
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Target: 5'- cGCAUCUuuacCGCCCaUAGCAccgaugauaagcauaAGCCCCa -3' miRNA: 3'- -CGUAGGuua-GCGGGgGUCGU---------------UCGGGG- -5' |
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| 32289 | 3' | -57.3 | NC_007023.1 | + | 123920 | 0.71 | 0.632635 |
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Target: 5'- uGCAcagCGAcCGCCCagagaaccaCCAGUAAGCCCCa -3' miRNA: 3'- -CGUag-GUUaGCGGG---------GGUCGUUCGGGG- -5' |
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| 32289 | 3' | -57.3 | NC_007023.1 | + | 83722 | 1.14 | 0.001111 |
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Target: 5'- cGCAUCCAAUCGCCCCCAGCAAGCCCCg -3' miRNA: 3'- -CGUAGGUUAGCGGGGGUCGUUCGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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