Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32291 | 3' | -49.3 | NC_007023.1 | + | 142408 | 0.66 | 0.999051 |
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Target: 5'- gAACCugGUuaUGCUCGU--UGGAACGg- -3' miRNA: 3'- -UUGGugCA--AUGGGCGaaAUCUUGCag -5' |
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| 32291 | 3' | -49.3 | NC_007023.1 | + | 115299 | 0.67 | 0.996581 |
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Target: 5'- gAACaucgaACgGUUGcCCCGCUUUAGggUGUg -3' miRNA: 3'- -UUGg----UG-CAAU-GGGCGAAAUCuuGCAg -5' |
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| 32291 | 3' | -49.3 | NC_007023.1 | + | 29107 | 0.67 | 0.995976 |
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Target: 5'- uGACUuuaUUACCCGCUUcgcGAGCGUCc -3' miRNA: 3'- -UUGGugcAAUGGGCGAAau-CUUGCAG- -5' |
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| 32291 | 3' | -49.3 | NC_007023.1 | + | 2949 | 0.67 | 0.995779 |
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Target: 5'- -uCCAUGUUACCCGCUac-GAuaugaccaucaaucGCGUUa -3' miRNA: 3'- uuGGUGCAAUGGGCGAaauCU--------------UGCAG- -5' |
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| 32291 | 3' | -49.3 | NC_007023.1 | + | 171614 | 0.69 | 0.988886 |
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Target: 5'- cAACCACGUUAaaCGUUacUGGGGCGUUg -3' miRNA: 3'- -UUGGUGCAAUggGCGAa-AUCUUGCAG- -5' |
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| 32291 | 3' | -49.3 | NC_007023.1 | + | 104752 | 1.07 | 0.017755 |
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Target: 5'- gAACCACGUUACCCGCUUUAGAACGUCc -3' miRNA: 3'- -UUGGUGCAAUGGGCGAAAUCUUGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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