Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32291 | 5' | -48.3 | NC_007023.1 | + | 118359 | 0.66 | 0.999902 |
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Target: 5'- gGGAAGGUG-UUGGCGGuauuaaaGAgcauACCGAUa- -3' miRNA: 3'- -CCUUCUACuAAUCGCC-------CU----UGGCUAgg -5' |
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| 32291 | 5' | -48.3 | NC_007023.1 | + | 159827 | 0.66 | 0.999875 |
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Target: 5'- aGAGGAUGAaaaaaugauGUGGGAACCugGAUUa -3' miRNA: 3'- cCUUCUACUaau------CGCCCUUGG--CUAGg -5' |
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| 32291 | 5' | -48.3 | NC_007023.1 | + | 55114 | 0.66 | 0.999875 |
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Target: 5'- uGGAGAaGA--AGCGGGuuCCG-UCCg -3' miRNA: 3'- cCUUCUaCUaaUCGCCCuuGGCuAGG- -5' |
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| 32291 | 5' | -48.3 | NC_007023.1 | + | 161915 | 0.66 | 0.99984 |
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Target: 5'- -cAGGAUGAUUGGUGcGGuaaAACgGAUCg -3' miRNA: 3'- ccUUCUACUAAUCGC-CC---UUGgCUAGg -5' |
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| 32291 | 5' | -48.3 | NC_007023.1 | + | 154080 | 0.66 | 0.999739 |
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Target: 5'- -aAAGgcGA--AGaCGGGaAGCCGAUCCu -3' miRNA: 3'- ccUUCuaCUaaUC-GCCC-UUGGCUAGG- -5' |
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| 32291 | 5' | -48.3 | NC_007023.1 | + | 175128 | 0.68 | 0.998832 |
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Target: 5'- cGAAGAUGA-UGGCGGGu--UGAaaaUCCg -3' miRNA: 3'- cCUUCUACUaAUCGCCCuugGCU---AGG- -5' |
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| 32291 | 5' | -48.3 | NC_007023.1 | + | 161631 | 0.72 | 0.980141 |
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Target: 5'- cGucGAUGAUUuGCGGGAACUGGgugUUCa -3' miRNA: 3'- cCuuCUACUAAuCGCCCUUGGCU---AGG- -5' |
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| 32291 | 5' | -48.3 | NC_007023.1 | + | 104787 | 1.09 | 0.022381 |
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Target: 5'- aGGAGAUGAUUAGCGGGAACCGAUCCc -3' miRNA: 3'- cCUUCUACUAAUCGCCCUUGGCUAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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