Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32292 | 5' | -47.9 | NC_007023.1 | + | 132637 | 0.67 | 0.998953 |
Target: 5'- ---uAAGCCGUAUgCCuCGCGAUGa--- -3' miRNA: 3'- uuucUUCGGCAUA-GG-GCGCUAUaauu -5' |
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32292 | 5' | -47.9 | NC_007023.1 | + | 60345 | 0.68 | 0.996189 |
Target: 5'- uGAAGAAGUCacgAUCCCGCGucuggGUUAu -3' miRNA: 3'- -UUUCUUCGGca-UAGGGCGCua---UAAUu -5' |
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32292 | 5' | -47.9 | NC_007023.1 | + | 47651 | 0.68 | 0.996189 |
Target: 5'- --uGAAGCgGUAUCCUGUGAg----- -3' miRNA: 3'- uuuCUUCGgCAUAGGGCGCUauaauu -5' |
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32292 | 5' | -47.9 | NC_007023.1 | + | 52276 | 0.69 | 0.994739 |
Target: 5'- gAAAGAAGCCucuaugugggcGUGUCgUGCGGUGUUc- -3' miRNA: 3'- -UUUCUUCGG-----------CAUAGgGCGCUAUAAuu -5' |
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32292 | 5' | -47.9 | NC_007023.1 | + | 99382 | 0.69 | 0.994739 |
Target: 5'- cAAAGAAcaaaaCGUAUUUCGCGAUAUUAAa -3' miRNA: 3'- -UUUCUUcg---GCAUAGGGCGCUAUAAUU- -5' |
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32292 | 5' | -47.9 | NC_007023.1 | + | 134728 | 1.03 | 0.039959 |
Target: 5'- uAAGAAGCCGUAUCCCGCGAUAUUAAg -3' miRNA: 3'- uUUCUUCGGCAUAGGGCGCUAUAAUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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