miRNA: 3'- aauaugcacAGGgACCACCUAGAUGC- -5'
miRNA: 3'- aauauGCACA--GGGACCACCUAGAUgc -5'
miRNA: 3'- aAUAUGCACaGGGACcACCU---AGAUGC- -5'
miRNA: 3'- -AAUAUGCACAGGGACCACCUAGAUGC- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32293 | 3' | -50.9 | NC_007023.1 | + | 143273 | 0.66 | 0.996528 |
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Target: 5'- --------uUCCgUGGUGGAUCUAUGa -3' miRNA: 3'- aauaugcacAGGgACCACCUAGAUGC- -5' |
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| 32293 | 3' | -50.9 | NC_007023.1 | + | 109430 | 0.67 | 0.988922 |
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Target: 5'- ----uUGUGUugCUCUGGUGGAUCUGu- -3' miRNA: 3'- aauauGCACA--GGGACCACCUAGAUgc -5' |
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| 32293 | 3' | -50.9 | NC_007023.1 | + | 96172 | 0.69 | 0.962574 |
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Target: 5'- cUAUGCGUGaCCUUGcUGGGgaaUCUGCGg -3' miRNA: 3'- aAUAUGCACaGGGACcACCU---AGAUGC- -5' |
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| 32293 | 3' | -50.9 | NC_007023.1 | + | 141967 | 1.08 | 0.01188 |
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Target: 5'- aUUAUACGUGUCCCUGGUGGAUCUACGa -3' miRNA: 3'- -AAUAUGCACAGGGACCACCUAGAUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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