Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 323 | 3' | -52.2 | AC_000010.1 | + | 17990 | 0.66 | 0.753366 |
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Target: 5'- cGGCGGCGGguccAGGCGgcuguuuaUCUGUUUCUGCAc -3' miRNA: 3'- -UCGCCGCU----UCUGUg-------AGGUAAAGGUGU- -5' |
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| 323 | 3' | -52.2 | AC_000010.1 | + | 13996 | 0.66 | 0.731172 |
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Target: 5'- cGGCGGCGgcGAUGCagCCA---CCACu -3' miRNA: 3'- -UCGCCGCuuCUGUGa-GGUaaaGGUGu -5' |
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| 323 | 3' | -52.2 | AC_000010.1 | + | 25502 | 0.66 | 0.7199 |
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Target: 5'- cAGCGGCaguGGCACUcaagcaggCCAUcUCCGCu -3' miRNA: 3'- -UCGCCGcuuCUGUGA--------GGUAaAGGUGu -5' |
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| 323 | 3' | -52.2 | AC_000010.1 | + | 4098 | 0.67 | 0.697077 |
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Target: 5'- cGGgGGUGGAGAUAgCUCCAUUgaagagCCuCAu -3' miRNA: 3'- -UCgCCGCUUCUGU-GAGGUAAa-----GGuGU- -5' |
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| 323 | 3' | -52.2 | AC_000010.1 | + | 25558 | 0.68 | 0.603976 |
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Target: 5'- cAGCGGCGAAGGCGaugggUCUuc-UCCugGg -3' miRNA: 3'- -UCGCCGCUUCUGUg----AGGuaaAGGugU- -5' |
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| 323 | 3' | -52.2 | AC_000010.1 | + | 15908 | 0.73 | 0.359892 |
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Target: 5'- gAGCaGGCGGAGACGCaucgCCAggcgCCACc -3' miRNA: 3'- -UCG-CCGCUUCUGUGa---GGUaaa-GGUGu -5' |
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| 323 | 3' | -52.2 | AC_000010.1 | + | 24842 | 1.09 | 0.001034 |
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Target: 5'- aAGCGGCGAAGACACUCCAUUUCCACAg -3' miRNA: 3'- -UCGCCGCUUCUGUGAGGUAAAGGUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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